Synaptotagmin1影响小鼠卵母细胞纺锤体组装和稳定  

Synaptotagmin1 regulates spindle assembly and during mouse oocyte meiotic maturation

作　　者：朱秀兰[1] 许虹[1] 罗燕群[1] 樊琳[1] 张春晖[2] 刘风华[1] 

机构地区：[1]广州医科大学附属广东省妇儿医院生殖健康与不孕症科,广东广州510000 [2]北京大学深圳医院生殖医学科,广东深圳518000

出　　处：《吉林医学》2016年第12期2837-2840,共4页Jilin Medical Journal

基　　金：国家自然科学基金[项目编号:81300479];深圳市科创委面上项目[项目编号:JCY20120616144719076]

摘　　要：目的:确定Synaptotagmin1(Syt1)对小鼠卵细胞纺锤体组装和稳定的影响。方法:免疫荧光法检测Syt1在小鼠卵母细胞发育不同时期的亚细胞定位。用紫杉醇处理小鼠MⅠ期卵母细胞,明确Syt1与微管动力的关系。注射Syt1特定的寡聚核苷酸降调卵母细胞中Syt1的表达,免疫荧光法检测Syt1降调后卵母细胞纺锤体的形态变化、染色体异常以及中心体蛋白γ-tubulin定位变化。结果:Syt1在卵母细胞减数分裂成熟的不同发育阶段都有表达,主要集中分布皮质区和MⅠ和MⅡ期纺锤体的两极。Syt1在MⅠ和MⅡ期与中心体相关蛋白γ-tubulin共定位。紫杉醇实验发现Syt1集中定位于星体和高度集中的微管两极。降调Syt1表达后,染色体排列紊乱,纺锤体组装异常比例升高,且大多数为无极、单极或者多级纺锤体,其次有拉长纺锤体和形态各异纺锤体。并影响微管组织中心相关蛋白γ-tubulin的正确定位。结论:Syt1可能作为一种微管组织中心相关蛋白调节纺锤体的组装稳定。Objective To identify the role of Sytl in regulating meiotic spindle assembly. Method Sytl localization was determined by immunofluorescence and confocal microscopy. The roles of Sytl in oocyte meiotic spindle and microtubule were analysed by morpholino interference,Taxol treatment and confocal microscopy. Results The results showed that Sytl was mainly accumulated at the oocyte cortex,strikingly at the spindle poles in both M Ⅰ and M Ⅱ phases and colocalization with ＂y - tubulin. Sytl was detected at the abnormal spindle poles as well as in the cytoplasmic asters after taxol treatment. It also showed that knockdown of Sytl caused abnormal spindles and misaligned chromosomes and disturbed the localization of γ - tubu- lin. Conclusion Sytl may act as a centrosome - associated protein to regulate spindle assembly during mouse oocyte meiotic maturation.

关 键 词：Synaptotagmin1 纺锤体 卵母细胞 微管组织中心 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]