人Mcl-1基因启动子区结合蛋白的生物信息学分析  

Bioinformatics Analysis of Transcription Factor Binding Site in the Promoter of Human Mcl-1 Gene

作　　者：杜冠魁[1] 骆凯丽 庞驰[1] 郭杨[1] 恽枫林 柳红琴 文茂羽 张雪子 肖曼[1] 

机构地区：[1]海南医学院理学院生化与分子生物学教研室,海南海口571199

出　　处：《吉林医学》2016年第12期2865-2867,共3页Jilin Medical Journal

基　　金：2013年国家自然科学基金[项目编号:81360359]

摘　　要：目的:对人Mcl-1基因启动子区结合蛋白进行生物信息学分析。方法:从Gene Bank数据库中获取人Mcl-1基因5'调控区,使用在线软件JASPAR对该序列可能存在的转录因子结合位点进行预测。结果:Relative profile score threshold在85%、90%、95%和100%时,该序列存在1700、1147、368和32个可能的转录因子结合位点,包含Arid3a,Atoh1,ETV6,Gata1,GATA3,KLF5,LMX1A,LMX1B,MZF1,Prrx2,SPI1,TBX4,USF1,YY1,ZEB1等。结论:人Mcl-1基因5'调控区存在多种转录因子的结合位点,这些转录因子大多与肿瘤发生,红细胞生成及神经发育等有关。Objective To analysis of transcription factor binding site in the promoter of human Mcl - 1 gene. Method The 5＇ regulatory region of Mcl - 1 gene was obtained from GeneBank database. The transcriptional binding sites of Mcl - 1 gene were predicted by online software JASpAR. Results 1700,1147,368 and 32 potential transcription factors binding sites were predicted in the 5＇ regulatory region of Mcl - 1 gene when the relative profile score higher than 85% ,90% ,95 % and 100% ,including Arid3a, Atohl, ETV6, Gatal, GATA3, KLFS, LMX1A, LMX1B, MZF1, Prrx2, SPI1, TBX4, USF1, YY1, ZEB1 and et al. Conclusion There are many transcription factor binding sites of human Mcl - i gene 5＇ regulatory region, which are associated with regulation of tumor occurrence, erythrocytopoiesis and neurodevelopment.

关 键 词：人Mcl-1基因 启动子5'调控区 转录因子结合位点 

分 类 号：R346[医药卫生—基础医学]