右美托咪定预处理对大鼠心肌缺血／再灌注损伤时心肌组织高迁移率族蛋白B1的影响  

Effects of dexmedetomidine preconditioning on myocardial expression of high mobility group box-1 protein during myocardial ischemia/reperfnsion injury in rats

作　　者：张静静[1] 嵇富海[1] 孟晓文[1] 王丽娜[1] 彭科[1] 朱亚娟[1] 

机构地区：[1]苏州大学附属第一医院麻醉科,215006

出　　处：《国际麻醉学与复苏杂志》2016年第12期1063-1067,共5页International Journal of Anesthesiology and Resuscitation

基　　金：国家自然科学基金（81471835,81471889）;苏州市科技发展计划（SYSD2013076）

摘　　要：目的探讨右美托咪定（dexmedetomidine，Dex）预处理对大鼠心肌缺血，再灌注损伤（myocardial ischemia／reperfusion injury，MI／RI）时心肌组织高迁移率族蛋白B1（highmobility group box-1 protein，HMGB1）表达量的影响。方法健康雄性SD大鼠52只，体重250～300g，按随机数字表法分为4组（每组13只）：假手术组（S组）、缺血/再灌注（ischemia／reperfusion，VR）组（I／R组）、Dex＋I／R组（D组）、Dex＋育亨宾＋I，R组（D／Y组）。结扎左冠状动脉前降支30min，恢复灌注120min制备MI／RI模型。再灌120min时采血ELISA法测定血清中IL-6、TNF-α浓度，随后处死大鼠摘取心脏，用2，3，5-氯化三苯基四氮唑（2，3，5-triphenyltetrazolium chloride，TIC）染色法测定心肌梗死面积，Western blot法测定心肌组织HMGB1蛋白的表达量。结果与S组比较，I／R组血清中IL-6、TNF-α含量[（336±41）、（636±25）ng／L]均显著增高（P〈0．05），心肌梗死面积明显增大（P〈0．05），心肌组织HMGB1蛋白表达量明显升高（P〈0．05）；与I／R组比较，D组血清中IL-6、TNF咀含量[（153±10）、（233±9）ng／L]均明显降低（P〈0．05），心肌梗死面积明显减少（P〈0．05），心肌组织HMGB1蛋白表达量显著降低（P〈0．05）；与D组比较，D／Y组血清中IL-6、TNF-α含量[（230±20）、（386±32）ng／L]均显著增高（P〈0．05），心肌梗死面积明显增大（P〈0．05），心肌组织HMGB1蛋白表达量明显升高（P〈0．05）。结论Dex预处理减轻MI／RI心肌组织HMGB1的表达量，减轻I／R损伤的炎症反应。Dex通过α2受体发挥保护作用。Objective To investigate the effects of dexmedetomidine （Dex） preconditioning on myocardial expression of high mobility group box-1 protein （HMGB1） during ischemia/reperfusion （I/R） injury in rats. Methods Fifty-two healthy male SD rats, weighing 250-300 g, were randomly divided into 4 groups（n=13）： group sham operation（group S）, group I/R, group Dex＋I/R（group D）, group Dex＋yohimbine＋I/R （group D/Y）. In group I/R, group D and group D/Y, myocardial I/R was produced by occlusion of left coronary anterior descending artery for 30 min followed by 120 min reperfusion. Blood samples were taken for detecting the concentration of IL-6 and TNF-α by ELISA at the end of reperfusion. The rats were then killed, and the heart was removed for measuring infarct size and area at risk using 2, 3, 5-triphenyhetrazolium chloride （TYC） staining. Myocardial HMGB1 expressions were measured by Western blot analysis. Results Compared with group S, the levels of IL-6[（336±41） ng/L], TNF-α[（636±25） ng/L] in serum and the IS/AAR ratio were significantly increased, the expression of HMGB1 were also significantly increased in group I/R （P〈0.05）. Compared with group I/R, the levels of IL-6 [（153±10） ng/L], TNF-α [（233±9） ng/L] in serum and the IS/AAR ratio were significantly decreased, and the expression of HMGBI were also significantly decreased in group D（P〈0.05）. Compared with group D, the levels of IL-6[（230±20） ng/L], TNF-α[（386±32） ng/L] in serum and the IS/AAR ratio were significantly increased, the expression of HMGB1 were also significantly increased in group D/Y （P〈0.05）. There was no significant difference in the above changes between group I/R and D/Y （P〉0.05）. Conclusions Dex preconditioning decrease the expression of HMGB1 and alleviate inflammatory response during myocardial ischemia/reperfusion injury （MI/RI） in rats, which might be a result of the α2 adrenergic receptor stimulation.

关 键 词：右美托咪定 预处理 心肌缺血/再灌注损伤 高迁移率族蛋白B1 大鼠 

分 类 号：R614[医药卫生—麻醉学]