曲尼司特对犬自体静脉移植术后再狭窄的影响  

Effect of tranilast on restenosis after canine autologous vein transplantation

作　　者：马英初 孙成林[2] 

机构地区：[1]沈阳医学院附属中心医院放射科,辽宁沈阳110024 [2]沈阳医学院附属中心医院普外一科,辽宁沈阳110024

出　　处：《实用手外科杂志》2016年第4期469-471,共3页Journal of Practical Hand Surgery

基　　金：辽宁省自然基金项目（项目编号：2015010381-301）

摘　　要：目的 观察曲尼司特对犬自体静脉移植术后再狭窄的影响.方法 将犬颈静脉外侧支间置移植于颈总动脉.术前一周开始用药至取材,实验组每日给予曲尼司特50 mg/kg.术后2周取材测定局部血管糜酶mRNA水平,应用病理形态学、计算机图像定量分析及增殖细胞核抗原（PCNA）免疫组织化学等方法观察其对自体移植静脉术后再狭窄的影响.结果 静脉移植术后局部血管糜酶mRNA活性表达明显增加,而曲尼司特可以显著抑制糜酶mRNA的表达（P〈0.05）;静脉移植术后静脉内膜的平均厚度、PCNA表达实验组均减少,与对照组相比差异有显著性（P〈0.05）.结论 曲尼司特通过糜酶途径对犬自体静脉移植术后再狭窄具有抑制作用.Objective To investigate the effect of tranilast on restenosis after canine autologous vein transplantation. Methods Transplanted the canine autologous internal jugular vein to common carotid artery by end to end bypass. The treated animals were given 50 mg/kg of tranilast daily since one week before the operation. At two week postoperatively, the grafts were harvested for analysis of chymase mRNA expression level、 intimal hyperplasia with pathological morphology and PCNA immunohistochemistry. The intimal hyperplasia was measured with a computerized image analysis system. Results Chymase mRNA expression of local blood vessels after venous transplant-ation increased significantly, and tranilast can significantly inhibit chymase mRNA expression（P〈0.05）; there was significant difference （P〈0.05） in the degree of intimal hyperplasia and the number of PCNA positive cells between the treated group and the control group. Conclusion Suppression of the chymase-dependent forming pathway may contribute to the effect of antirestenosis of canine vein autografts.

关 键 词：糜酶 曲尼司特 移植静脉 再狭窄 

分 类 号：R622.4[医药卫生—整形外科]