肝细胞生长因子对光老化成纤维细胞凋亡及周期的影响  

Effect of hepatocyte growth factor on apoptosis and cell cycle of skin fibroblasts induced by UV

作　　者：杨清建 毕波[1] 朱宁文[2] 陈亮[1] 贾传龙 卢勇舟 周轶群 杨平[1] 郭妤 朱晶晶[1] 刘天一[1] YANG Qing-jian BI Bo ZHU Ning-wen CHEN Liang JIA Chuan-long LU Yong-zhou ZHOU Yi-qun YANG Ping GUO Yu ZHU ring-ring LIU Tian-yi(Department of Plastic Surgery, Huadong Hospital Affiliated to Fudan University, Shanghai 200040, Chin)

机构地区：[1]复旦大学附属华东医院整形外科,上海200040 [2]复旦大学附属华山医院皮肤科

出　　处：《中国美容整形外科杂志》2016年第12期740-743,共4页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：国家自然科学基金(81272125,81301642);上海市卫生系统优秀学科带头人培养计划(XBR2011033);863项目(SS2014AA020705)

摘　　要：目的探讨肝细胞生长因子(HGF)对紫外线诱导皮肤成纤维细胞周期及凋亡的影响。方法建立光老化模型,皮肤成纤维细胞(FBs)经HGF处理后行倒置显微镜观察、细胞计数并予以流式细胞周期检测;以HGF预处理皮肤FBs 2 h,中波紫外线(UVB)照射诱导体外FBs细胞发生细胞损伤,Tunel染色试剂盒检测细胞凋亡。结果光老化模型建立后,UVB+HGF组细胞存活较UVB组增多(P<0.05);HGF减少光老化成纤维细胞P53的表达,改善光老化FBs细胞周期分布;HGF能够减少UVB导致的FBs凋亡(P<0.05)。结论 HGF可调控光老化细胞周期分布及减少细胞凋亡,在光老化治疗中具有潜在应用前景。Objective To investigate the influence of HGF on cell cycle and apoptosis in photoaging skin fibroblasts induced by UV. Methods A photoaging model was established, skin FBs were treated by HGF under inverted microscope, cell count and flow cytometry were detected. HGF pretreatment skin FBs for 2 hours, FBs in cells in vitro induced by UVB irradiation injury, Apoptosis was detected by Tunel staining kits. Results After photoaging model establishment, the UVB ＋ HGF group had more surviving cells than that in the UVB group （ P 〈 0.05 ） ; HGF reduces the expression of P53 in photoaging fibroblasts, and improves the distribution of photoaging FBs cell cycle. HGF can also decrease FBs apoptosis caused by UVB exposure （ P 〈 0.05 ）. Conclusion HGF can regulate photoaging FBs cell cycle distribution and reduce cell apoptosis, which has a potential application prospect in photoaging treatment.

关 键 词：肝细胞生长因子 光老化 成纤维细胞 细胞周期 凋亡 

分 类 号：R758.1[医药卫生—皮肤病学与性病学]