生物反应器-微载体技术培养Vero细胞制备柯萨奇病毒A组16型  

Preparation of Coxsackievirus A16 by culture of Vero cells using bioreactor-microcarrier technology

作　　者：刘志成[1] 卿琰 岳凤先[1] 井敏敏 黄芬[1] 井申荣[1] LIU Zhi-cheng QING Yan YUE Feng-xian JING Min-min HUANG Fen JING Shen-rong(Medical Faculty of Kunming University of Science and Technology, Kunming 650500, Yunnan Province, Chin)

机构地区：[1]昆明理工大学医学院,云南昆明650500

出　　处：《中国生物制品学杂志》2017年第1期79-81,共3页Chinese Journal of Biologicals

摘　　要：目的利用生物反应器-微载体培养技术培养Vero细胞制备柯萨奇病毒A组16型(Coxsackievirus A16,CA16)。方法应用生物反应器-微载体培养法进行Vero细胞培养,待细胞长成致密单层时,接种CA16,于37℃培养,每隔24 h观察细胞病变情况,并检测病毒滴度。结果 Vero细胞在微载体上吸附140 h后,绝大部分细胞在微载体上长成致密单层,细胞密度约为12.3×10~5个/ml;接种CA16后72 h,Vero细胞完全病变,几乎全部从微载体上脱落,病毒滴度达最高,约7.75 TCID_(50)/ml。结论成功采用生物反应器-微载体培养技术培养Vero细胞制备了CA16,且病毒滴度较高,为CA16灭活疫苗的研制奠定了基础。Objective To prepare Coxsackievirus A16（CA16） by culture of Vero cells using bioreactor-microcarrier technology. Methods Vero cells were cultured by using bioreactor-microcarrier technology, inoculated with CA16 after a dense monolayer was formed, and cultured at 37 ℃. CPE was observed every 24 h, while the virus titer was determined.Results Most of Vero cells formed a dense monolayer 140 h after adsorption onto microcarriers, of which the density was about 12. 3×10~5 cells/ml. Complete CPE was observed 72 h after inoculation with CA16, while almost all the cells detached from the microcarrier, and the virus titer reached a peak value of about 7. 75 TCID_（50）/ml. Conclusion CA16 was prepared by culture of Vero cells using bioreactor-microcarrier technology, which reached a high titer. It laid a foundation of preparation of inactivated CA16 vaccine.

关 键 词：生物反应器 微载体 VERO细胞 柯萨奇病毒A组16型 

分 类 号：R373.23[医药卫生—病原生物学] R392-33[医药卫生—基础医学]