Effects of electronically stimulating Tianshu(ST 25) and Dachangshu(BL 25), Quchi(LI 11) and Shangjuxu(ST 37) on the expressions of jejunum c-kit protein and c-kit m RNA in rats with functional diarrhea  

作　　者：Wang Yuan Niu Wenmin Yang Xiaohang Liu Na Liu Zhibin 

机构地区：[1]Combination of Acupuncture and Medicine Innovation Research Center, Shaanxi University of Chinese Medicine [2]Combination of Acupuncture and Medicine Innovation Research Center,Shaanxi University of Chinese Medicine [3]Department of orthopedics, Red Cross Hospital Affiliated to Xi'an Jiaotong University

出　　处：《Journal of Traditional Chinese Medicine》2016年第6期779-783,共5页中医杂志（英文版）

基　　金：Supported by National Key Basic Research Program of China(973 Program):Bidirectional Regulation Effect and Mechanism of Acupuncture on Different Acupoints on Functional Bowel Disorders(No.2011CB505204)

摘　　要：OBJECTIVE: To investigate the effects of electronically stimulating Tianshu(ST 25) and Dachangshu(BL 25), Quchi(LI 11) and Shangjuxu(ST 37) on the jejunum c-kit protein and c-kit m RNA in rats with functional diarrhea(FD).METHODS: FD models were established through intragastric administration with folium sennae. Experimental rats were then divided into 4 groups:blank group, model group, electroacupuncture group Ⅰ [Tianshu(ST 25) and Dachangshu(BL 25)of both sides] and electroacupuncture group Ⅱ [Quchi(Li 11) and Shangjuxu(ST 37) ofboth sides], 10 in each. After treatment with electroacupuncture for 10 days, The expressions of jejunum c-kit protein and c-kit m RNA in each group were detected with Western blot and Real-Time quantitative real-time polymerase chain reaction(PCR).RESULTS: The expressions of c-kit protein and c-kit m RNA in the model group increased significantly compared to those in the blank group(P < 0.01);the expressions in electroacupuncture group Ⅰsignificantly decreased compared to those in the model group(P < 0.01).CONCLUSION: Our findings suggest that electronically stimulating both Tianshu(ST 25) and Dachangshu(BL 25) significantly increased the expressions of jejunum c-kit protein and c-kit m RNA in FD rats, which means the treatment might have better therapeutic effects on FD.OBJECTIVE： To investigate the effects of electronically stimulating Tianshu（ST 25） and Dachangshu（BL 25）, Quchi（LI 11） and Shangjuxu（ST 37） on the jejunum c-kit protein and c-kit m RNA in rats with functional diarrhea（FD）.METHODS： FD models were established through intragastric administration with folium sennae. Experimental rats were then divided into 4 groups：blank group, model group, electroacupuncture group Ⅰ [Tianshu（ST 25） and Dachangshu（BL 25）of both sides] and electroacupuncture group Ⅱ [Quchi（Li 11） and Shangjuxu（ST 37） ofboth sides], 10 in each. After treatment with electroacupuncture for 10 days, The expressions of jejunum c-kit protein and c-kit m RNA in each group were detected with Western blot and Real-Time quantitative real-time polymerase chain reaction（PCR）.RESULTS： The expressions of c-kit protein and c-kit m RNA in the model group increased significantly compared to those in the blank group（P 〈 0.01）;the expressions in electroacupuncture group Ⅰsignificantly decreased compared to those in the model group（P 〈 0.01）.CONCLUSION： Our findings suggest that electronically stimulating both Tianshu（ST 25） and Dachangshu（BL 25） significantly increased the expressions of jejunum c-kit protein and c-kit m RNA in FD rats, which means the treatment might have better therapeutic effects on FD.

关 键 词：Diarrhea Interstitial cells of Cajal Proto-oncogene proteins c-kit Electroacupuncture 

分 类 号：R245[医药卫生—针灸推拿学]