Effect of moxa smoke produced during combustion of Aiye(Folium Artemisiae Argyi) on behavioral changes in mice inhaling the smoke  

作　　者：Yang Jia Zheng Xiaojun Jin Ran Han Li Ha Lue Li Jun Wang Lei Liu Ping Chu Zhusheng Huang Chang Chang Hongsheng Lao Lixing Zhao Baixiao 

机构地区：[1]School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China [2]Encephalopathy Department of Traditional Chinese Medicine Hospital, Baoji 721000, China [3]Acupuncture Department of South District of Guanganmen Hospital Affiliated to the China Academy of Chinese Medical Sciences, Beijing 102618, China [4]Institute of Health Care of Beijing University of Chinese Medicine, Beijing 100029, China [5]Health Administration of Beijing Electric Power Hospital, Beijing 100073, China [6]Department of Students' Affairs of Zhejiang Chinese Medical University, Hangzhou 310053, China [7]School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China [8]School of Chinese Medicine, Hong Kong University, Hong Kong 999077, China

出　　处：《Journal of Traditional Chinese Medicine》2016年第6期805-811,共7页中医杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China:Effect of Moxibustion on"Longevity Gene"SIRT1 Regulation in Aged Mice(No.81403449);NSFC Project:Effect of Moxibustion and its Therapeutic Factors on Mitochondrial Energy Metabolism and Apotosis Pathyway in APP/PS1Transgenic Alzheimer's Disease Mouse Model(No.81574068);National Program on Key Basic Research Project(973 Program):the Study of Components and Mechanisms of Artemisia and the Product and Moxibustion and Safety Evaluation(No.2009CB522906)

摘　　要：OBJECTIVE: To evaluate the effects that inhalation of the combustion products of the traditional Chinese herb Aiye(Folium Artemisiae Argyi) has on the central nervous system.METHODS: Forty Kunming mice(half male) were randomly assigned(n = 10/group) to a control group(C) or one of three moxa smoke concentration groups(% opacity): low(L1; 0.4%), medium(M1; 2%), and high(H1; 15%). Mice in the latter three groups were exposed to moxa smoke in a dynamic gas exposure cabinet for 20 min per day for7 days. Mice in control group were placed in the same cabinet without any intervention. For the sleep experiments, another 50 mice were divided into five groups of 10 mice each: a saline-injected control group, L1 + pentobarbital sodium(PS)-injected group, M1+PS group, H1(15%)+PS group,and a positive control group(10 mg/kg, chlorpromazine, p.o.). The weight, general activities, locomotor activities, rotarod performance, sleep duration,and sleeping rate induced by a subthreshold dose of pentobarbital sodium were recorded in the mice,and the composition of moxa smoke was analyzed using headspace gas chromatography(GC-HS).RESULTS: A low concentration of smoke significantly decreased the frequency of locomotor activities and the time for which the mice remaining on the rotarod; however, a high smoke concentration significantly prolonged the pentobarbital-induced sleeping time and sleeping rate.CONCLUSION: The concentration-dependent relaxing effects of moxa smoke on the Central Nervous System(CNS) were confirmed. Moreover, GC-HS analysis showed that the component present in the highest concentration in moxa smoke was eucalyp-tol, an essential oil well recognized for its soothing effects on the CNS. This may therefore be accountable for the sedative effects of moxa smoke.OBJECTIVE： To evaluate the effects that inhalation of the combustion products of the traditional Chinese herb Aiye（Folium Artemisiae Argyi） has on the central nervous system.METHODS： Forty Kunming mice（half male） were randomly assigned（n = 10/group） to a control group（C） or one of three moxa smoke concentration groups（% opacity）： low（L1; 0.4%）, medium（M1; 2%）, and high（H1; 15%）. Mice in the latter three groups were exposed to moxa smoke in a dynamic gas exposure cabinet for 20 min per day for7 days. Mice in control group were placed in the same cabinet without any intervention. For the sleep experiments, another 50 mice were divided into five groups of 10 mice each： a saline-injected control group, L1 ＋ pentobarbital sodium（PS）-injected group, M1＋PS group, H1（15%）＋PS group,and a positive control group（10 mg/kg, chlorpromazine, p.o.）. The weight, general activities, locomotor activities, rotarod performance, sleep duration,and sleeping rate induced by a subthreshold dose of pentobarbital sodium were recorded in the mice,and the composition of moxa smoke was analyzed using headspace gas chromatography（GC-HS）.RESULTS： A low concentration of smoke significantly decreased the frequency of locomotor activities and the time for which the mice remaining on the rotarod; however, a high smoke concentration significantly prolonged the pentobarbital-induced sleeping time and sleeping rate.CONCLUSION： The concentration-dependent relaxing effects of moxa smoke on the Central Nervous System（CNS） were confirmed. Moreover, GC-HS analysis showed that the component present in the highest concentration in moxa smoke was eucalyp-tol, an essential oil well recognized for its soothing effects on the CNS. This may therefore be accountable for the sedative effects of moxa smoke.

关 键 词：Artemisia Argyi Moxa smoke Central nervous system INHALATION 

分 类 号：R245.8[医药卫生—针灸推拿学]