二陈加桃红四物汤改善类风湿关节炎的机理研究  

Mechanisms Behind the Therapeutic Effects of Er Chen Combined with Tao Hong Si Wu Tang(ECSWT) on Rheumatoid Arthritis

作　　者：王腾腾[1,2] 赵永见[1,2] 陈岩[1,2] 李金龙[1,2] 王拥军[1,2] 施杞[1,2] 梁倩倩[1,2] 徐浩[1,2] Wang Tengteng Zhao Yongjian Chen Yan Li Jinlong Wang Yongjun Shi Qi Liang Qianqian Xu Hao(Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China Institute of Spine Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China)

机构地区：[1]上海中医药大学附属龙华医院,上海200032 [2]上海中医药大学脊柱病研究所,上海200032

出　　处：《世界科学技术-中医药现代化》2016年第11期1874-1878,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委重点项目(81330085):痰瘀型类风湿性关节炎与淋巴关系的基础研究;负责人:施杞;国家自然科学基金委重大国际合作项目(81220108027):益气祛湿法调控淋巴系统对炎症性关节炎的影响;负责人:王拥军;教育部高等学校全国优秀博士学位论文作者专项资金资助项目(201276):负责人:梁倩倩;上海市龙华医院龙医创新团队(LYCX-01):祛瘀化瘀防治颈痛和炎性关节炎的基础研究;负责人:梁倩倩;上海市卫生与计划生育委员会中医药三年行动计划项目(ZY3-CCCX-2-1002):国家中医临床研究基地建设项目;负责人:崔学军;筋骨理论与治法教育部重点实验室;负责人:王拥军;上海市长宁区卫生与计划生育委员会"光华卓越PI工程"项目(2016-01);负责人:施杞;上海市教育委员会高校实验技术队伍建设计划(LH01.24.001):负责人:赵永见

摘　　要：目的:类风湿关节炎(Rheumatoid Arthritis,RA)是一种累及全身小关节的自身免疫性疾病。前期研究发现,充足的淋巴回流功能有利于RA的恢复。本文主要探讨二陈加桃红四物汤治疗本病的疗效及其对淋巴管回流功能的影响。方法:本研究采用10周龄TNF转基因(TNF-Tg)小鼠为模型,以二陈加桃红四物汤灌胃治疗12周,并选取同窝野生型小鼠作对照,采用Micro-CT观测小鼠的踝关节骨量,采用近红外-吲哚菁绿系统检测小鼠下肢淋巴管功能。结果:Micro-CT三维成像显示,与对照组相比,TNF-Tg组可见明显的骨质破坏,踝关节结构严重受损。二陈加桃红四物汤治疗后,骨结构得到了一定程度的保护。淋巴管回流功能检测显示,与对照组相比,TNF-Tg组的淋巴管清除率明显下降;与TNF-Tg组相比,二陈加四物汤可促使受损的淋巴功能恢复。与对照组小鼠相比,TNF-Tg组的Pulse值明显下降,与TNF-Tg组相比,二陈加桃红四物汤可促进Pulse值明显升高。结论:二陈加桃红四物汤可以有效促进TNF转基因小鼠模型下肢的淋巴回流功能,从而缓解关节炎症,保护骨组织免受侵蚀。Rheumatoid arthritis（RA） is a chronic autoimmune disorder primarily occurred in small joints. Our previous studies suggested that sufficient lymphatic drainage was favorable for the recovery of RA. This study aimed at exploring the effect of Er Chen combined with Tao Hong Si Wu Tang（ECSWT） on RA in TNF transgenic（TNF-Tg） mice. Ten-week old TNF-Tg mice and WT littermates were detected with indocyanine green-nearinfrared（ICG-NIR） lymphatic imaging system before and after accepting the 12-week intragastric administration of ECSWT. All ankle joints were assessed by micro-CT scanning. According to three dimensional images of Micro-CT, it was found that the ankle joints in the TNF-Tg group were much eroded compared with the control group. The bone mass and structure were protected after the treatment of ECSWT. ICG-NIR results showed that lymphatic clearance rate of the TNF-Tg group decreased compared with those of the control group. In comparison with the TNF-Tg group, ECSWT promoted the repair of lymphatic function. Compared with the control group, the pulse value of the TNF-Tg group declined; while this condition could be rescued by ERSWT. In conclusion, ECSWT mitigated bone erosion of astragalus bone area in TNF-Tg mice in contrast to the saline mice, while promoted the pulse value and clearance of lymphatic vessels afferent from footpad to popliteal lymph node, implying that ECSWT was a promising agent for treating RA through its promoting lymphatic drainage function effects.

关 键 词：类风湿关节炎 二陈加桃红四物汤 TNF转基因小鼠 淋巴回流功能 

分 类 号：R274[医药卫生—中医骨伤科学]