羟基红花黄色素A与芍药苷联合用药对大鼠脑缺血再灌注损伤的保护作用  

Synergistic protective effect of hydroxy safflower yellow A and paeoniflorin on cerebral ischemia reperfusion injury in rats

作　　者：秦莎莎[1] 余梦黎 廖金明[3] 徐愉林 郑丽娴[3] 朱原[3] 李蕾[1] 姚晖[4] 张继平[4] 韩彬[1] 

机构地区：[1]广东药科大学中药学院,广东广州510006 [2]广东医科大学药学院,广东湛江524023 [3]南方医科大学中医药学院,广东广州510515 [4]南方医科大学附属佛山医院,广东佛山528000

出　　处：《广东药学院学报》2016年第6期747-751,761,共6页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省自然科学基金项目(S2013010012284);广东省中医药局课题(20152074);佛山市医学重点专科培育项目(Fspy2-2015008)

摘　　要：目的研究羟基红花黄色素A(HSYA)与芍药苷联合用药对急性局灶性脑缺血再灌注(I/R)损伤大鼠的协同保护作用,并探讨其作用机制。方法 110只雄性SD大鼠,随机分为6组:HSYA组(5.0 mg/kg)、芍药苷组(5.0 mg/kg)、HSYA与芍药苷联合用药组(合用组,各5.0 mg/kg)、银杏内酯组(5 mg/kg)、模型组和假手术组。采用改良线栓法复制大鼠大脑中动脉栓塞(MCAO)再灌注模型。脑缺血1 h再灌注6 h后进行神经功能缺失评分及尾静脉注射给药;给药7 d后进行神经功能缺失评分;氯化三苯基四氮唑(TTC)染色法检测脑梗死面积;免疫组化(IHC)法检测磷酸化Akt1蛋白的表达量的变化情况。结果与假手术组比较,模型组大鼠治疗前评分明显高于假手术组,差异具有统计学意义(P<0.01),说明造模成功;与模型组比较,银杏内酯组、合用组、芍药苷组和HSYA组能不同程度地抑制大鼠体质量下降(P<0.05,P<0.01),改善脑缺血再灌注大鼠的神经功能缺失症状(P<0.05,P<0.01),降低皮质区神经细胞的损伤程度,减小脑梗死面积(P<0.05,P<0.01),促进磷酸化Akt1蛋白的表达(P<0.01);HSYA组、芍药苷组、银杏内酯组磷酸化Akt1蛋白的表达较合用组低(P<0.01)。结论 HSYA与芍药苷联合用药对脑缺血再灌注大鼠的脑损伤具有一定的协同保护作用,二者联合用药的作用优于单独用药,可能与二者联合用药发挥活血化瘀与神经保护作用有关。Objective To study the synergistic protection and mechanism of hydroxy safflower yellow A（ HSYA） and paeoniflorin in acute focal cerebral ischemia reperfusion injury in rats. Methods 110 male SD rats were randomly divided into six groups, including HSYA group（ 5. 0 mg / kg）, paeoniflorin group（ 5.0 mg / kg）,HSYA combined with paeoniflorin group（ 5.0 mg / kg）,gingko lactone group（ 5.0 mg / kg）,the model group and the sham group. The model of rat middle cerebral artery occlusion（ MCAO） was replicated by modified thread embolism method. Nerve function loss was scored and drugs were administrated by intravenous injection after 1 h cerebral ischemia and 6 h reperfusion. Neurological deficit was revalued after7 day treatment. The area of cerebral infarction was detected by triphenyl tetrazolium chloride（ TTC）staining. The expression of phosphorylated Akt1 protein was identified by immunohistochemistry（ IHC）.Results Compared with the sham group, the scores of the pre-treatment rats in the operation group were significantly higher than those in the sham-operation group（ P〈0. 01）, indicating that the model was successful. Compared with the model group, the loss of body weight, neurological deficits, cortical cell damage and cerebral infarction area were all attenuated, but the expression of phosphorylated Akt1 was increased in four drug-treated groups（ P〈0.05 or P〈0.01）. Meanwhile,compared with the combination of HSYA and paeoniflorin group,the expression of phosphorylated Akt1 was lower in other three groups（ P〈0.01）. Conclusion HSYA combined with paeoniflorin displays a synergistic protection against cerebral ischemia and reperfusion injury,which is better than single drug treatment.

关 键 词：脑缺血再灌注损伤 羟基红花黄色素A 芍药苷 

分 类 号：R285.5[医药卫生—中药学]