精脒对SW620细胞增殖及糖酵解的影响  

Effect of spermidine on the growth and glycolysis of SW620 cells

作　　者：张芬[1] 吴燕燕[1] 臧林泉[1] 潘雪刁[1] 王桂香[1] 

机构地区：[1]广东药科大学药科学院,广东广州510006

出　　处：《广东药学院学报》2016年第6期767-770,789,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金项目(81302895;81102465)

摘　　要：目的探讨精脒对人结肠癌SW620细胞增殖及糖酵解的影响。方法以SW620细胞为研究对象,用0.625~2.5μmo L/L精脒（SPD）作用于细胞,细胞密度以104个/m L接种于96孔培养板,分别培养24 h后,MTT法检测细胞增殖情况;同时,取6孔板培养24 h的细胞上清液,用试剂盒检测SW620细胞葡萄糖消耗及乳酸水平;细胞密度以1.2×10~5个/m L接种于6孔培养板中,培养24 h后加入不同浓度SPD,继续培养24 h后,Western blot检测缺氧诱导因子（HIF-1）、乳酸脱氢酶（LDHA）及葡萄糖转运蛋白1（GLUT1）表达的变化。结果与对照组比较,精脒各浓度组均能促进SW620细胞的增殖（P〈0.01）,且促进作用呈剂量依赖性;精脒各浓度组作用后SW620细胞内葡萄糖的消耗及乳酸含量明显增多（P〈0.01）,呈剂量依赖性;与对照组比较,LDHA、HIF-1及GLUT1蛋白表达水平随着精脒浓度的升高而增加（P〈0.01）,且呈剂量依赖性。结论精脒具有促进人结肠癌SW620细胞增殖及糖酵解的作用。Objective To investigate the effect of spermidine（ SPD） on the growth and glycolysis of SW620 cells. Methods SW620 cells were cultured in vitro routinely with different concentrations（ 0. 625- 2. 5μmol /L）. Cell proliferation was detected by MTT. Glucose consumption and lactate concentrations were detected by test kit. The expression of HIF-1, LDHA and GLUT1 was detected by western blot. Results Compared with the control group, SPD promoted the proliferation of SW620 cells（ P〈0. 01）. SPD with different concentrations increased the glucose consumption and lactate concentrations in SW620 cells（ P〈0.01）. Meantime, the expression of HIF-1, LDHA and GLUT1 was increased with the increased concentrations of SPD（ P〈0.01）. Conclusion SPD could promote the proliferation and glycolysis of SW620 cells.

关 键 词：精脒 SW620细胞 细胞增殖 糖酵解 

分 类 号：R96[医药卫生—药理学]