银屑病患者皮肤组织信号转导和转录激活因子3基因的表达及其意义  

Expression and Significance of Signal Transducer and Activator of Transcription 3 in Psoriatic Skin

作　　者：王丽丽[1] 王也[2] 王英[1] 王猛[3] 白彦萍[1] WANG Li-li WANG Ye WANG Yin WANG Meng BAI Yah-ping(Department of Dermatology and Venerology, China-Japan Friendship Hospital, Graduate school of Peking Union Medical College, Beijing, 100029, China Department of Pathology, China-Japan Friendship Hospital, Beijing, 100029, China Research Laboratory of Pathophysiology, Graduate school of Peking Union Medical College, China-Japan Friendship Hospital, Beijing, 100029, China)

机构地区：[1]北京协和医学院研究生院,中日友好临床医学研究所皮肤病与性病科,北京100029 [2]中日友好医院病理科,北京100029 [3]北京协和医学院研究生院,中日友好临床医学研究所病理生理研究所,北京100029

出　　处：《现代生物医学进展》2016年第36期7015-7017,7033,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81373636)

摘　　要：目的:检测寻常型银屑病患者皮损区及非皮损区信号转导和转录激活因子3(STAT3)m RNA的表达差异,并探讨其与银屑病皮损发生的关系。方法:采用实时定量PCR方法检测13例寻常型银屑病患者皮损区和非皮损区组织中STAT3基因的m RNA表达水平,并用免疫组织化学方法同时检测两种磷酸化STAT3蛋白在皮损区、非皮损区的表达,比较其差异。结果:寻常型银屑病患者皮损区STAT3基因的m RNA表达水平明显高于非皮损区(P值为0.0001)。两种磷酸化STAT3蛋白均主要表达在角质形成细胞的细胞核,皮损区的表达水平均明显高于非皮损区(P均<0.05),且均可见在真皮浅层血管内皮细胞、淋巴细胞中的表达。结论:STAT3基因在银屑病皮损的角质形成细胞中的m RNA表达显著上调,两种磷酸化STAT3蛋白在皮损区的表达水平均高于非皮损区,提示其参与了银屑病的病理生理过程。Objective： To investigate the expression of signal transducer and activator of transcription 3 （STAT3） in the skin of psoriatic patients and its relationship with psoriasis. Methods： The mRNA expression level of STAT3 in lesional and non-lesional skin of 13 psoriatic patients were evaluated using real-time quantitative PCR technique while the expressions of two kinds of phosphorylated STAT3 were detected by immunohistochemistry. Results： Compared with non-lesional psoriatic skin, the mRNA expression level of STAT3 was significantly higher in the lesional psoriatic skin （P=0.0001）. Immunohistochemistry showed stronger expression of both two kinds of phosphorylated STAT3 in nucleus of keratinocytes in lesional skin relative to non-lesional psoriatic skin and showed high expression in dermal microvascular endothelial cells and surrounding lymphocytes. Conclusion： The mRNA expression of STAT3 and the expression ofphosphorylated STAT3 were significantly higher in the lesional psoriatic skin than non-lesional psoriatic skin, implying that STAT3 might be involved in the pathogenesis of psoriasis.

关 键 词：银屑病 信号转导和转录激活因子3 

分 类 号：R758.63[医药卫生—皮肤病学与性病学] Q522[医药卫生—临床医学]