Characterization of two monoclonal antibodies, 38F10 and 44D11, against the major envelope fusion protein of Helicoverpa armigera nucleopolyhedrovirus  

作　　者：Zijiao Zou Jinliang Liu Zhiying Wang Fei Deng HuaUn Wang Zhihong Hu Manli Wang Tao Zhang Zijiao Zou Jinliang Liu Zhiying Wang Fei Deng HuaUn Wang Zhihong Hu Manli Wang Tao Zhang(State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China)

机构地区：State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

出　　处：《Virologica Sinica》2016年第6期490-499,共10页中国病毒学（英文版）

基　　金：supported by the grants from the National Science Foundation of China (No. 31370191 and 31621061);the Strategic Priority Research Program of the Chinese Academy of Sciences (grant XDB11030400);Open Research Fund Program of the Key Laboratory of Agricultural and Environmental Microbiology, Chinese Academy of Sciences

摘　　要：The envelope fusion protein F of baculoviruses is a class I viral fusion protein which play a significant role during virus entry into insect cells. F is initially synthesized as a precursor（F_0） and then cleaved into a disulfide-linked F_1 and F_2 subunits during the process of protein maturation and secretion. To facilitate further investigation into the structure and function of F protein during virus infection, monoclonal antibodies（mAbs） against the F_2 subunit of Helicoverpa armigera nucleopolyhedrovirus（HearNPV）（Ha F） were generated. Two kinds of mAbs were obtained according to their different recognition epitopes： one kind of mAbs, as represented by 38F10,recognizes amino acid（aa） 85 to 123 of F_2 and the other kind, represented by 44D11, recognizes aa148 to 173 of F_2. Western blot and immunofluorescence assay confirmed that both of the mAbs recognized the F protein expressed in HearNPV infected cells, however, only 44D11 could neutralize HearNPV infection. The results further showed that 44D11 may not interact with a receptor binding epitope, rather it was demonstrated to inhibit syncytium formation in cells expressing the Ha F protein. The results imply that the monoclonal antibody 44D11 recognizes a region within HaF_2 that may be involved in the F-mediated membrane fusion process. 基金The envelope fusion protein F of baculoviruses is a class I viral fusion protein which play a significant role during virus entry into insect cells. F is initially synthesized as a precursor(F_0) and then cleaved into a disulfide-linked F_1 and F_2 subunits during the process of protein maturation and secretion. To facilitate further investigation into the structure and function of F protein during virus infection, monoclonal antibodies(mAbs) against the F_2 subunit of Helicoverpa armigera nucleopolyhedrovirus(HearNPV)(Ha F) were generated. Two kinds of mAbs were obtained according to their different recognition epitopes: one kind of mAbs, as represented by 38F10,recognizes amino acid(aa) 85 to 123 of F_2 and the other kind, represented by 44D11, recognizes aa148 to 173 of F_2. Western blot and immunofluorescence assay confirmed that both of the mAbs recognized the F protein expressed in HearNPV infected cells, however, only 44D11 could neutralize HearNPV infection. The results further showed that 44D11 may not interact with a receptor binding epitope, rather it was demonstrated to inhibit syncytium formation in cells expressing the Ha F protein. The results imply that the monoclonal antibody 44D11 recognizes a region within HaF_2 that may be involved in the F-mediated membrane fusion process.

关 键 词：HearNPV F protein monoclonal antibody 38F10  44Dll  epitope neutralizingactivity membrane fusion 

分 类 号：S476[农业科学—农业昆虫与害虫防治]