玉米Zma-miR169i启动子的克隆及分析  

Cloning and Analysis of Zma-miR169i Promoter in Zea mays

作　　者：丑晓红[1] 王春燕[2] Chou Xiaohong Wang Chunyan(Inner Mongolia Technical College of Mechanics and Electrics, Huhhot, 010010 College of Food Engineering, Jilin Engineering Normal University, Changchun, 130052)

机构地区：[1]内蒙古机电职业技术学院,呼和浩特010010 [2]吉林工程技术师范学院食品工程学院,长春130052

出　　处：《分子植物育种》2017年第1期17-22,共6页Molecular Plant Breeding

摘　　要：采用荧光定量PCR分析表明Zma-miR169i受干旱胁迫和脱落酸诱导表达。以玉米叶片基因组DNA为材料,根据预测的Zma-miR169i启动子序列设计引物,采用PCR方法克隆得到Zma-miR169i上游一段长度为1 827 bp的启动子序列。利用启动子预测网站Plant CARE分析发现,Zma-miR169i启动子序列具有CAAT-box、TATA-box基本的顺式作用元件和一些参与植物激素和非生物胁迫应答的相关顺式作用元件。研究结果为进一步分析Zma-miR169i的功能奠定了基础。The research by using the realtime fluores-cence quantitative PCR analysis showed that the Zma-miR169i was induced by drought stress, and abscisic acid（ABA） treatment. Based on the predicted sequence of Zma-miR169i promoter region, genomic DNA extraction of maize was used as material, a 1 827 bp fragment was cloned from the genomic DNA of the maize by PCR method. Plant CARE analysis showed that the promoter sequence contained basic elements TATA-box, CAAT-box and some cis-acting elements involved in response to abiotic stresses and plant hormones. These results provided reference for further analysis on the functional characterization of Zma-miR169i.

关 键 词：玉米 Zma-miR169i 启动子 

分 类 号：Q943.2[生物学—植物学] S513[农业科学—作物学]