杜仲愈伤组织中类胡萝卜素合成相关基因的表达分析  

Expression Analysis of the Related Genes of Carotenoid Biosynthesis in Eucommia ulmoides Calli

作　　者：张俊娥[1] 郑文强[1] Zhang Jun＇e Zheng Wenqiang(College of Life Science, Jiangxi Normal University, Nanchang, 33002)

机构地区：[1]江西师范大学生命科学学院,南昌330022

出　　处：《分子植物育种》2017年第1期107-112,共6页Molecular Plant Breeding

基　　金：国家自然科学基金(31100396);江西省教育厅科学技术研究项目(GJJ150332);江西省亚热带植物资源保护和利用重点实验室开放基金(YRD201404)共同资助

摘　　要：通过对杜仲愈伤组织转录组高通量测序结果进行KEGG通路深入分析发现,强光(光强为12 000 Lx,16 h光照,8 h黑暗)培养杜仲愈伤组织18 d,黑暗培养作为对照,与类胡萝卜素合成相关的七种酶(九个基因)上调表达,七种酶分别为9-顺式-环氧类胡萝卜素双加氧酶(EC 1.13.11.51,9-cis-epoxycarotenoid dioxygenase),脱落酸8-羟化酶(EC 1.14.13.93,(+)-abscisic acid 8'-hydroxylase),紫黄质脱环氧化酶(EC 1.10.99.3,violaxanthin de-epoxidase),15-顺式-八氢番茄红素合成酶(EC 2.5.1.32,15-cis-phytoene synthase),番茄红素环化酶(Cru A),β-胡萝卜素羟化酶(Crt R-b),八氢番茄红素脱饱和酶(Crtl-e)。qRT-PCR分析表明,杜仲愈伤组织中与类胡萝卜素合成相关的九个基因的表达与其转录组高通量测序结果一致,均上调表达。由此推断,强光促进杜仲愈伤组织中类胡萝卜素的积累。本研究可以为获取高产类胡萝卜素资源和杜仲遗传分析提供有价值的资料。An in-depth analysis of KEGG pathway on transcriptome high-throughput sequencing results of Eucommia ulmoides calli indicated that the seven enzymes（nine genes） related to carotenoid biosynthesis were up-regulated while the calli were cultured for 18 d（light intensity is 12 000 Lx, 16 h light, 8 h dark）. The seven enzymes were EC 1.13.11.51, 9-cis-epoxycarotenoid dioxygenase, EC 1.14.13.93,（＋）-abscisic acid 8’-hydroxylase, EC1.10.99.3, violaxanthin de-epoxidase, EC 2.5.1.32, 15-cis-phytoene synthase, Cru A, Crt R-b and Crtl-e. The analysis of qRT-PCR showed that the expression of the nine genes related to carotenoid biosynthesis was consistent with the results of the high throughput sequencing, which were up-regulated expression. It was inferred that strong light promoted the carotenoids accumulation in Eucommia ulmoides calli. This study can provide valuable data for obtaining high yield of carotenoids and genetic analysis resources of Eucommia ulmoides.

关 键 词：杜仲 愈伤组织 类胡萝卜素 实时荧光定量PCR 

分 类 号：S567.19[农业科学—中草药栽培] Q943.2[农业科学—作物学]