南方型紫花苜蓿耐盐突变体叶片盐胁迫应答转录因子鉴定与分析  

Identification and Characterization of Salt-responsive Transcription Factors in Leaves of Southern Type Alfalfa

作　　者：裴翠明 张振亚[1] 马进[1] Pei Cuiming Zhang Zhenya Ma Jin(Zhejiang Agriculture and Forestry University, Faculty of Landscape Architecture, Lin＇an, 311300)

机构地区：[1]浙江农林大学风景园林与建筑学院,临安311300

出　　处：《分子植物育种》2017年第1期315-321,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31272494);浙江省自然科学基金项目(LY16C170003)共同资助

摘　　要：转录因子可以调节众多下游基因的表达,在植物抗逆境中起重要的调节作用。以南方型紫花苜蓿Medicago sativa‘Millennium’的耐盐突变体为材料,以正常培养(MT_CK2)和盐胁迫(MT_N2)条件下的2个样品叶片进行转录组测序分析,并进行qRT-PCR验证RNA-Seq结果的可靠性,研究耐盐突变体叶片盐胁迫应答相关转录因子基因。结果表明:经过250 mmol/L NaCl胁迫72 h,共检测到30 900个基因表达量发生了变化,7 694个基因差异表达,共有隶属于50个转录家族的422个转录因子发生了差异表达,上调表达268个,下调表达154个。盐胁迫应答基因数量最多的是MYB基因家族,其次是WRKY、NAC、b HLH和AP2-EREBP转录家族。此外,候选出MsANT、Msb HLH36、MsNAI1、Msb ZIP、Msb ZIP73A、MsC3H、MsMYB85、MsNAD、MsMYB、MsNAC、MsTrihelix和MsWRKY等与盐胁迫应答相关的重要转录因子。本研究为揭示紫花苜蓿盐胁迫分子机制奠定基础。Transcription factors（TFs） can regulate many downstream genes expression and play an important role in plant stress responses. To explore salt stress response related TFs genes, two leaves samples were analyzed RNA-sequencing under the condition of MT_CK2 and MT_N2 using salt stress, with southern type of alfalfa（Medicago sativa） ‘Millennium’salt tolerantce mutant as materials, and verify the reliability of RNA-Seq results by qRT-PCR. The results showed that, through 250 mmol/L NaCl stressed 72 h, 30 900 genes were detected expression changes, 7 694 of which were thought different expression genes. Among these genes, 422 TFs belonging to 50 TF families expressed differentially, 268 were up-regulated and 154 were down-regulated. In all TF families, genes with salt stress-responsive from the MYB family were observed most, followed by WRKY,NAC, b HLH and AP2-EREBP families. In addition, candidate genes such as MsANT、Msb HLH36、MsNAI1、Msb ZIP、Msb ZIP73A、MsC3H、MsMYB85、MsNAD、MsMYB、MsNAC、MsTrihelix and MsWRKY may be important TFs in salt stress responses were identified. This study was helpful to reveal the further research on the molecular mechanism of salt stress in alfalfa.

关 键 词：南方型紫花苜蓿 转录因子 叶片 盐胁迫 

分 类 号：S541.9[农业科学—作物学] Q943.2[生物学—植物学]