c-FLIP_L在白血病中的表达及临床意义  

Expression of c-FLIP_L in Leukemia and Its Clinical Significance

作　　者：迟昨非[1] 何秋颖[1] 杨威[2] 傅煜[3] 符爽 庄倩[1] 

机构地区：[1]中国医科大学附属盛京医院小儿血液内科,沈阳110022 [2]中国医科大学附属盛京医院第二血液内科,沈阳110022 [3]中国医科大学附属盛京医院血液研究室,沈阳110022

出　　处：《中国医科大学学报》2017年第2期120-125,共6页Journal of China Medical University

基　　金：沈阳市科技计划(F13-316-1-08)

摘　　要：目的探讨c-FLIPL在不同类型白血病中的表达水平及其临床意义。方法应用实时PCR检测103例不同类型白血病患者骨髓单个核细胞c-FLIP_L mRNA表达,包括急性淋巴细胞白血病(ALL)54例(其中初诊37例、复发5例、完全缓解12例)、急性髓细胞白血病(AML)38例(其中初诊24例、复发6例、完全缓解8例)、初诊急性未分化白血病(AUL)2例、初诊慢性粒细胞白血病(CML)6例、初诊慢性粒单核细胞白血病(CMML)3例。应用流式细胞术对所选病例进行免疫表型检测。结果在初诊和复发的白血病中c-FLIP_L mRNA呈异常高表达趋势,二者间差异无统计学意义(P>0.05)。初诊AUL及初诊CML的c-FLIP_L mRNA表达高于其他初诊组,但差异无统计学意义(P>0.05)。初诊ALL、AML、AUL、CML、CMML与对照组、完全缓解组比较,c-FLIP_L mRNA的差异均有统计学意义(P<0.05)。c-FLIP_L mRNA表达与患者危险度分级、初诊白细胞数、血乳酸脱氢酶(LDH)、血羟丁酸脱氢酶(HBDH)、CD45、融合基因TEL-AML1相关,与患者年龄、性别、血纤维蛋白原及染色体异常无关。结论白血病患者c-FLIP_L mRNA表达异常增高,并与危险度分级、初诊白细胞数、血LDH、血HBDH及预后密切相关。Objective To investigate the expression of c-FLIPL in leukemia and explore its clinical significance. Methods The expression level of c-FLIPLmRNA in bone marrow mononuclear cells was determined by real-time polymerase chain reaction （PCR） in 103 leukemia patients with different types of leukemia, including 54 cases of acute lymphocytic leukemia （ALL） with 37 newly diagnosed, 5 relapsed, and 12 complete remission, 38 cases of acute myeloid leukemia （AML） with 24 newly diagnosed, 6 relapsed, and 8 complete remission, newly diagnosed 2 cases of acute undifferentiated leukemia （AUL）, 6 cases of chronic myelocyfic leukemia （CML）, and 3 cases of chronic myelomonocyfie leukemia （CM- ML）. The immunophenotype of patients were detected by flow cytometry. Results Expression level of c-FLIPL mRNA was higher in ncwly diagnosed and relapsed leukemia patients. There was no significant difference between newly diagnosed and relapsed leukemia patients （ P〉 0.05 ）. Expression level of c-FLIPL mRNA of AUL and CML was higher than that in other patients, but there was no significant difference （P 〉 0.05 ）. Expression level of c-FLIPL mRNA of all newly diagnosed and relapsed leukemia patients was significantly higher than that in control group and complete remission group （P 〈 0.05 ）. The expression level of c-FLIPL mRNA was correlated with risk stratification, white blood cell （WBC）, lactate dehydrogenase （LDH）, hydroxybutyrate dehydrogenase （HBDH）, CD45 and TEL-AML1, but was not associated with age, sex, tibrinogen and chromosome abnormality. Conclusion c-FLIPL mRNA is highly expressed in leukemia patients, and is closely related with risk stratification, WBC, LDH, HBDH and prognosis.

关 键 词：c—FLIPL 白血病 买时PCR 

分 类 号：R552[医药卫生—血液循环系统疾病]