不同工艺提取龙血竭中紫檀茋的含量测定  

Determination of pterostilbene in Dragon's Blood with different extraction

作　　者：胡迎庆[1] 韩慧文[1] 宋月英[1] 邓昌沪 

机构地区：[1]中国人民武装警察部队医学院,天津300162 [2]厦门今润丰华医药科学有限公司

出　　处：《中成药》2002年第8期616-618,共3页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目 (3 0 0 70 0 88)

摘　　要：目的 :测定不同工艺提取的龙血竭中紫檀的含量。方法 :以C18反相键合硅胶为固定相 ,乙腈 1%冰醋酸 (41∶5 9)为流动相 ,检测波长为 319nm ,用外标法定量。结果 :紫檀在 10 .4～ 10 4ng范围有良好线性关系 ,r =0 .9992 ,方法回收率为 98.76 % ,RSD为 1.74 %。新型的免加热提取工艺的龙血竭中紫檀的含量低于加热提取工艺龙血竭中紫檀的含量。结论 :免加热提取龙血竭明显优于加热提取工艺。Objective: To establish HPLC method for determination of pterostilbene in Dragon's Blood with different extraction technology. Method: Applying a C 18 phase column and acetonitrile 1% acetic acid(41∶59) as mobile phase, detecting at 319nm and quantitating with external standard method. Results: The standard curves of pterostilbene was linear in the concentration range of 10.4～104ng, r =0.9992. The average recovery was 98.28%, RSD =1.97%. The content of pterostilbene in Dragon's Blood extracted with ordinary temperature was lower than that with heating technology. Conclusion: The content of pterostilbene extracted with ordinary temperature extraction in Dragon's blood have the advantage of heating extraction.

关 键 词：HPLC 龙血竭 紫檀芪 提取工艺 含量测定 

分 类 号：R282.710.3[医药卫生—中药学]