牛膝水煎液及甾酮类组分对脂多糖诱导的RAW264.7细胞炎症因子的影响  

Effects of Achyranthesbidentata Blume and Steroid Components on Lipopolysaccharide- induced Inflammatory Factors in RAW264. 7 Cells

作　　者：闫佳驹[1] 孙海峰[1] 崔毅[2] 闫萍[1] Yan Jiaju Sun Haifeng Cui Yi Yan Ping(Heilongjiang University Of Chinese Medicine, Harbin, Heilongjiang 150040, China Farming Research Institute of Heilongjiang, Qiqihar Heilongjiang, 161005, China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江省畜牧研究所,黑龙江齐齐哈尔161005

出　　处：《现代畜牧科技》2017年第2期1-3,共3页Modern Animal Husbandry Science & Technology

基　　金：黑龙江省科技攻关计划(GC06C40504);黑龙江中医药大学校科研基金资助

摘　　要：目的:研究牛膝水煎液及甾酮类组分对脂多糖(LPS)诱导的RAW264.7巨噬细胞中炎症因子的影响,探讨牛膝及其甾酮类组分的抗炎作用。方法:体外培养RAW264.7细胞,采用MTT法检测牛膝水煎液及其甾酮类组分对RAW264.7细胞的存活率影响,Griess法测定细胞炎症反应产生的NO含量,ELISA法检测细胞上清液中IL-1β,TNF-α的含量。结果:牛膝水煎液及甾酮类组分能明显抑制LPS诱导的RAW264.7巨噬细胞NO的分泌(P<0.01),以及IL-1β(P<0.01),TNF-α(P<0.01)的表达,并呈现出良好的剂量依赖性。结论:牛膝水煎液及甾酮类组分在浓度小于500mg/L的范围内对RAW264.7细胞无显著毒性。牛膝水煎液及甾酮类组分可通过抑制细胞NO的分泌与IL-1β和TNF-α的表达而发挥抗炎作用。Objective： To observe the effect of inflammatory factors from decoction of Achyranthesbidentata Blume and its ketosteroids that LPS（ lipopolysaccharide） was induced by RAW264. 7 cells. Methods： The RAW264. 7 cells were cultivated in vivo,and used MTT methods to determine the cytotoxicity of decoction and its ketosteroids of Achyranthesbidentata Blume.Usegriess method to determine the NO content of the inflammatory reaction cell and use the method of enzyme linked immunosorbent（ ELISA） to detect tumor necrosis factor alpha（ TNF- α） and interleukin 1β（ IL- 1β） content in the supernatant fluid. Results： The decoction and its ketosteroids could inhibit LPS induced by RAW264. 7 cells releasing inflammatory factor NO（ P〈0. 01）,IL- 1β（ P 0. 01）,TNF- α（ P 0. 01）. Conclusion： The concentrations of decoction and its ketosteroids are less than 500 mg / L for RAW264. 7 cells and have no significant toxicity. The decoction and its ketosteroids may inhibit the release of cytokines NO,IL- 1β,TNF- α and exert its anti- inflammatory effects.

关 键 词：牛膝 甾酮 RAW264.7 炎症因子 

分 类 号：R282[医药卫生—中药学]