ATP适体接枝Fe_3O_4纳米粒子的制备及化学发光酶检测应用  

Nanoparticles Grafted by ATP Aptamer: Preparation and Application in Chemiluminescence Enzyme Analysis

作　　者：严喜鸾[1] 陈馨[1] 高文菊[1] 魏婷婷[1] 林月敏[1] 艾凡荣[2,3] 

机构地区：[1]南昌大学资源环境与化工学院,南昌330031 [2]南昌大学机电工程学院,南昌330031 [3]南昌大学转化医学研究院,南昌330031

出　　处：《无机化学学报》2017年第2期340-346,共7页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.81102289;51102131;31660491);江西省自然科学基金(No.20132BAB205106;20142BAB216033)资助项目

摘　　要：制备了油酸修饰的Fe_3O_4纳米粒子,利用盐酸多巴胺对其表面进行氨基化改性,制得水分散性良好的Fe_3O_4纳米粒子,用X射线衍射、透射电镜、傅里叶变换红外光谱仪、振动样品磁强计和紫外-可见吸收光谱进行表征。随后,将氨基修饰的三磷酸腺苷(ATP)适体接枝到Fe_3O_4纳米粒子上,结合荧光素酶化学发光法进行ATP的定量检测,并应用于市售酸奶中乳酸菌ATP含量的检测,其灵敏度高、重现性好。各项实验结果表明所制备的Fe_3O_4纳米粒子是一种分散性好、易分离的载体,其粒径均一、稳定、磁性强、与适体结合性能好,拓展了Fe_3O_4纳米粒子在分析检测领域的应用。Oleic acid-modified Fe3O4 nanoparticles was prepared and dopamine hydrochloride was chosen to amino-modify these nanoparticles. The Fe304 nanoparticles have good water-dispersible, and were characterized by X-ray diffraction （XRD）, transmission electron microscopy （TEM）, Fourier transformed infrared （FTIR） spectroscopy, vibrating sample magnetometer and uhravioletvisible spectroscopy （UV-Vis） spectra. Then, aminomodified adenosine triphosphate （ATP） aptamer was grafted onto Fe3O4 nanopartieles, and the amount of ATP was detected with luciferase chemiluminescence method. Meanwhile, the amount of ATP of lactic acid bacteria in commercially yogurt was tested, which had high sensitivity and good reproducibility. The results showed that these functionalized-Fe3O4 nanoparticles could act as good dispersion, easy separation carrier, which indicated uniform particle size, good stability, strong magnetism and excellent binding ability with ATP aptamer. The development of Fe3O4 nanoparticles largely expanded its application in the field of analysis.

关 键 词：FE3O4 纳米粒子 氨基功能化 适体 化学发光检测 

分 类 号：O614.811[理学—无机化学]