芪桂益脉灵对急性心肌缺血再灌注损伤保护机制的研究  

Protection mechanism of Qigui Yimailing for acute myocardial ischemia-reperfusion injury

作　　者：张超越[1] 赵龙[1] 徐京育[2] ZHANG Chaoyue ZHAO Long XU Jingyu(Heilongjiang University of Chinese Medicine, Harbin 150040, China The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China)

机构地区：[1]黑龙江中医药大学,哈尔滨150040 [2]黑龙江中医药大学附属第一医院,哈尔滨150040

出　　处：《吉林中医药》2017年第1期67-71,共5页Jilin Journal of Chinese Medicine

基　　金：黑龙江省自然科学基金项目(H201326)

摘　　要：目的通过测定ICAM-1、VCAM-1、PI-3Kp85am RNA和AKT2m RNA的表达,探讨芪桂益脉灵对急性心肌缺血再灌注损伤的保护机制。方法随机将50只SD大鼠分为5组,每组10只,每日清晨空腹灌胃1次,连续灌服7 d。空白对照组(灌服生理盐水)开胸暴露左冠状动脉后不做任何处理,假手术组(灌服生理盐水)开胸暴露左冠状动脉后穿线并结扎,单纯缺血再灌注组(灌服生理盐水)、芪桂益脉灵低剂量组(0.6 g/kg)和芪桂益脉灵高剂量组(1.2g/kg)结扎左冠状动脉前降支造成急性心肌梗死(AMI)模型。再灌注后腹主动脉采血5 m L,离心后将上层血清用双抗体夹心法酶联免疫测定ICAM-1和VCAM-1的表达;取大鼠心尖部缺血组织,采用半定量逆转录—聚合酶链反应(RT-PCR)方法来测定PI-3Kp85am RNA、AKT2m RNA的表达。结果芪桂益脉灵组与单纯缺血再灌注组比较,ICAM-1和VCAM-1表达均下降(P<0.05),PI3Kp85am RNA、AKT2m RNA水平明显升高(P<0.05),其中高剂量组的效果更加显著。结论芪桂益脉灵可减轻心肌缺血再灌注后的炎症反应,并且可激活再灌注损伤挽救激酶信号通路,从而保护血管内皮和受损心肌组织。Objective To discuss the protection mechanism of Qigui Yimailing to acute myocardial ischemia- reperfusion injury by measuring ICAM-1,VCAM-1,PI-3Kp85amRNA and AKT2mRNA expression. Mothods 50 SD male rats were randomly divided into 5 groups with 10 rats in each group. Whose irrigation were served 7 days in a row during the morning. The normal group（N） do any processing after exposing the left coronary artery .The sham operation group （SH）make ligation after exposing the left coronary artery.The pure ischemia reperfusion group, Qigui Yimailing low dose group（QL） and Qigui Yimailing high dose group（QH）,with the physiological saline, Qigui Yimailing （big ang small dose,l.2 g/kg and 0.6 g/kg） respectively. Acute myocardial infarction（AMI） models were caused by ligation of the left anterior descending branch of the coronary artery in the successful rat models＇ hearts 5 ml blood was produced from the abdominal aorta after reperfusion, upper serum with double antibody clip art enzyme-linked immune after the centrifugation was measured to express ICAM-1 and VCAM-1. RT-PCR was used to determine the expressions of PI3Kp85amRNA and AKT2mRNA in the tip of the rat cardiac ischemic tissue. Results Compared with Qigui Yimailing group and I/R group, ICAM-1 and VCAM-1 expressions decreased, PI3Kp85amRNA and AKT2mRNA levels increased significantly （P〈0.05）, and the effects of QH group were more significant. Conclusion Qigui Yimailing can reduce myocardial ischemia reperfusion inflammatory response, and can activate the reperfusion injury of rescue kinase signaling pathways,protect blood vessel endothelium and damaged heart tissue.

关 键 词：芪桂益脉灵 心肌缺血再灌注 ICAM-1 VCAM-1 PI3Kp85amRNA AKT2mRNA 

分 类 号：R542.2[医药卫生—心血管疾病] R285.5[医药卫生—内科学]