人参皂苷Rg_1配伍乌头碱对体外培养心衰模型心肌细胞的保护作用  

Protective Effect of Compatibilities of Ginsenosides Rg_1 and Aconitine on Myocardial Cells of in vitro Cultured Heart Failure Model

作　　者：董艳红[1,2] 谢晓芳[1,2] 李雪梅[1,2] 朱雅宁 彭成[1,2] DONG Yanhong XIE Xiaofang LI Xuemei ZHU Yaning PENG Cheng(College of Pharmacy, Cheng- du University of TCM, Chengdu 611137, China State Key Lab Breeding Base of Systematic Research Devel- opment and Utilization of Chinese Medicine Resources Co-founded by Sichuan Province and MOST, Chengdu 611137, China Huarun Sanjiu （Ya＇an）Pharmaceutical Co., Ltd., Sichuan Ya＇an 625099, China)

机构地区：[1]成都中医药大学药学院,成都611137 [2]中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137 [3]华润三九(雅安)药业有限公司,四川雅安625099

出　　处：《中国药房》2017年第4期472-475,共4页China Pharmacy

基　　金：国家自然科学基金重点项目(No.81630101);国家科技支撑计划课题(No.2011BAI13B05);"重大新药创制"科技重大专项(No.2013ZX09201018);四川省2013年重点科技自筹项目(No.2013JYZ010;2013SZ0114);四川省博士后科研项目

摘　　要：目的:考察人参皂苷Rg_1配伍乌头碱对体外培养心衰模型心肌细胞的保护作用。方法:将新生大鼠心肌细胞分为正常对照组、模型组、阳性对照组(去乙酰毛花苷注射液,1×10^(-7)mol/L)、人参皂苷Rg_1组(1×10^(-8)mol/L)、乌头碱组(1×10^(-9)mol/L)以及人参皂苷Rg_1与乌头碱不同配比的配伍组(1∶1、2∶1、1∶2,V/V)。除正常对照组外,其余各组细胞均以0.8%戊巴比妥钠诱导心肌细胞心衰模型。成模后,各组细胞给予相应药物培养1 h,然后检测细胞总三磷酸腺苷(ATP)酶、Ca^(2+)-Mg^(2+)-ATP酶和Na^+-K^+-ATP酶活力;检测细胞培养液中酸性磷酸酶(ACP)、乳酸脱氢酶(LDH)活力和脑钠肽(BNP)、肿瘤坏死因子α(TNF-α)含量以及细胞中总糖原含量。结果:与正常对照组比较,模型组细胞总ATP酶和Ca^(2+)-Mg^(2+)-ATP酶活力明显降低,Na^+-K^+-ATP酶活力明显升高,培养液中ACP、LDH活力及BNP含量均明显升高(P<0.05)。与模型组比较,各给药组细胞总ATP酶活力显著升高,细胞培养液中LDH活力显著降低,且以人参皂苷Rg1与乌头碱2∶1配伍时作用最强;乌头碱组和各配伍组细胞Na^+-K^+-ATP酶活力均显著降低,差异均有统计学意义(P<0.05);各给药组细胞培养液中ACP、BNP活力均有降低的趋势,细胞内总糖原含量和培养液中TNF-α含量无显著变化(P>0.05)。结论:人参皂苷Rg_1配伍乌头碱可改善大鼠心衰模型心肌细胞ATP酶活力和膜通透性,调节细胞分泌BNP,对心衰模型心肌细胞有一定保护作用,且以人参皂苷Rg_1与乌头碱2∶1配伍时作用最强。OBJECTIVE： To investigate the protective effect of the compatibilities of ginsenosides Rg1 and aconitine on myocar- dial cell of in vitro cultured heart failure model. METHODS： The myocardial cells of neonate rat were grouped into normal control group, model group, positive control group （Deslanoside injection, 1×10-7 mol/L）, ginsenosides Rg1 group （1×10-8 mol/L）, acon- itine group （1 × 10-9 mol/L） or their compatibilities groups （1 ： 1,2 ： 1,1 ： 2, V/V）. Except for normal control group, other groups were given 0.8% pentobarbital sodium to induce heart failure model of myocardial cells. After modeling, each group was given rele- vant medicine for 1 h, and then the activities of T-ATPase, Ca2＋-Mg2＋-ATPase, Na＋-K＋-ATPase in cells were all detected. The activi- ties of acyl carrier protein （ACP） and lactate dehydrogenase （LDH）, and the contents of brain natriuretic party（BNP）, TNF-α and total glycogen were measured in cell culture fluid. RESULTS： Compared with normal control group, T-ATPase and Ca2＋-Mg2＋-ATPase activities were decreased significantly in model group; meanwhile, Na＋-K＋-ATPase activity was increased signifi- cantly, and ACP, LDH activities and BNP content in cell culture fluid were increased significantly （P〈0.05）. Compared with mod- el group, the activities of T-ATPase in treatment groups were increased significantly, while the activity of LDH in cell culture fluid was decreased significantly; when the volume ratio of ginsenoside Rgl to aconitine was 2 ： 1, protective effect was the strongest; the activities of Na＋-K＋-ATPase in aconitine group and compatibility groups were all decreased significantly, with statistical signifi- cance （P〈0.05）. The activities of ACP and BNP in cell culture fluid were all decreased in treatment groups, but the content of to- tal glycogen in cells and the TNF-α content of in cell culture fluid had no change （P〉0.05）. CONCLUSIONS： Compatibility of ginsenosides Rg1 and aconitine can impro

关 键 词：人参皂苷Rg1 乌头碱 配伍 心衰模型 心肌细胞 细胞膜通透性 离子转运相关酶 

分 类 号：R285.5[医药卫生—中药学]