何首乌乙酸乙酯提取物对秀丽线虫的抗衰老作用研究  

Anti-aging Effect Study of Ethyl Acetate Extract from Folygonum multiflora on Caenorhabditis Elegans

作　　者：唐进法[1] 张帆[2] 李宇辉[2] 吴宿慧[2] 李寒冰[2] 李伟霞[1] 李学林[1] TANG Jinfa ZHANG Fan LI Yuhui WU Suhui LI Hanbing LI Weixia LI Xuelinl(Dept. of Pharmacy, the First Affiliated Hospital of Henan University of TCM, Zhengzhou 450000, China College of Pharmacy, Henan University of TCM, Zhengzhou 450000, China)

机构地区：[1]河南中医药大学第一附属医院药学部,郑州450000 [2]河南中医药大学药学院,郑州450000

出　　处：《中国药房》2017年第4期493-496,共4页China Pharmacy

基　　金：2015年度河南省中医药科学研究专项课题(No.2015ZY02101)

摘　　要：目的:研究何首乌乙酸乙酯提取物(EPM)对秀丽线虫(简称"线虫")寿命的影响,考察EPM抗衰老作用。方法:设立EPM低、中、高浓度组(25、37.5、50 mg/L,以生药计)和空白组(0 mg/L),考察EPM对线虫寿命的影响;设立EPM组(37.5 mg/L)和空白组(0 mg/L),分别进行线虫的生殖实验、急性热应激实验和急性氧化应激实验,考察EPM对线虫生殖能力以及压力应激的影响。结果:在寿命实验中,EPM低、中、高浓度组线虫的平均寿命较空白组均显著延长(P<0.05或P<0.01),以中浓度EPM作用最为明显;增殖实验中,EPM组线虫在第2、3天中的子代数量较空白组显著增加(P<0.05);在急性热应激实验和急性氧化应激实验中,EPM组线虫的平均生存时间较空白组明显延长(P<0.05)。结论:37.5 mg/L的EPM能延缓线虫衰老且不损害其生殖能力。OBJECTIVE： To study the lifespan effect of ethyl acetate extract from Polygonum multiflora （EPM） on caenorhab- ditis elegans, and to investigate its anti-aging effect. METHODS： EPM low-concentration, medium-concentration and high- concen- tration groups （25,37.5,50 mg/L, calculated by crude drug） and blank group （0 mg/L） were established to investigate the effects of EPM on the lifespan of caenorhabditis elegans. EPM group （37.5 mg/L） and blank group （0 mg/L） were established to perform re- productive test, acute heat stress test and acute oxidative stress test of caenorhabditis elegans. The effects of EPM on reproductive capacity and pressure stress of caenorhabditis elegans were investigated. RESULTS： The lifespan test, average lifespan of cae-norhabditis elegans in EPM low-concentration, medium-concentration and high-concentration groups were prolonged significantly, compared to blank group （P〈0.05 or P〈0.01）, especially in EPM medium-concentration group. In reproductive test, the number of offspring of caenorhabditis elegans in EPM group on the second and third day were increased significantly, compared to blank group （P〈0.05）. In acute heat stress test and acute oxidative stress test, average survival time of caenorhabditis elegans in EPM group was prolonged significantly （P〈0.05）. CONCLUSIONS： 37.5 mg/L EPM can retard the aging process of caenorhabditis ele- gans and doesn＇t damage the reproductive capacity.

关 键 词：何首乌 乙酸乙酯提取物 抗衰老 秀丽线虫 寿命 氧化应激 热应激 

分 类 号：R285[医药卫生—中药学]