尼美舒利联合奥沙利铂对食管癌模型大鼠微血管密度及免疫功能的影响  

Effects of Nimesulide Combined with Oxaliplatin on Microvessel Density and Immune Function of Esophageal Cancer Model Rats

作　　者：王丕明 冉旺旺 谭柏清 罗维晓 WANG Piming RAN Wangwang TAN Baiqing LUO Weixiao(Clinical Laboratory, Armed Police Shandong Corps Hospital, Jinan 250014, China Shandong Biobase Bio-industrial Co., Ltd., Jinan 250200, China)

机构地区：[1]武警山东省总队医院检验科,济南250014 [2]山东博科生物产业有限公司,济南250200

出　　处：《中国药房》2017年第4期505-508,共4页China Pharmacy

摘　　要：目的:研究尼美舒利联合奥沙利铂对食管癌模型大鼠微血管密度及免疫功能的影响。方法:取48只大鼠建立食管癌模型后随机分为模型组、奥沙利铂组、尼美舒利组和联用组,每组12只。建模后1 d,模型组大鼠尾iv 5%的葡萄糖注射液1 m L,每周3次,ig等量羧甲基纤维素钠溶液,每周1次;奥沙利铂组大鼠尾iv奥沙利铂13.6 mg/kg,每周3次;尼美舒利组大鼠ig尼美舒利20mg/kg,每周1次;联用组大鼠同法给予奥沙利铂和尼美舒利,给药时间为8周。观察各组大鼠肿瘤生长情况、肿瘤组织微血管密度、外周血免疫功能指标。结果:与模型组比较,各给药组大鼠肿瘤体积、瘤质量、肿瘤组织的积分吸光度和阳性血管密度均降低(P<0.05);奥沙利铂组和联用组大鼠外周血CD3^+、CD4^+T细胞数和CD4^+/CD8^+比值均降低,CD8^+T细胞数均增加(P<0.05)。与尼美舒利组比较,奥沙利铂组和联用组大鼠肿瘤体积、瘤质量、肿瘤组织的积分吸光度和阳性血管密度,以及外周血CD3^+、CD4^+T细胞数和CD4^+/CD8^+比值均降低,抑瘤率和CD8^+T细胞数均增加(P<0.05),其中联用组较奥沙利铂组更明显(P<0.05)。结论:奥沙利铂联合尼美舒利能够更有效地抑制食管癌的生长,其机制可能与抑制肿瘤组织中血管新生、增强免疫有关。OBJECTIVE： To study the effects of nimesulide combined with oxaliplatin on microvessel density and immune func- tion of esophageal cancer model rats. METHODS ： 48 rats were selected to establish esophageal cancer model and then randomly di- vided into model group, oxaliplatin group, nimesulide group and combination group, with 12 rats in each group. 1 d after model- ing. model group was given 5% Glucose injection 1 mL via tail vein, 3 times a week, and constant volume of Sodium carboxy- methylcellulose solution, once a week. Oxaliplatin group was given oxaliplatin 13.6 mg/kg via tail vein, 3 times a week. Nimesu- lide group was given nimesulide 20 mg/kg intragastrically, once a week. Combination group was given oxaliplatin and nimesulide with same usage as above. The administration lasted for 8 weeks. The cancer growth, microvessel density of cancer tissue, peripher- al blood immune function index were observed in four groups. RESULTS： Compared with model group, cancer size, cancer weight, integral absorbance of cancer and positive vessel density were all decreased in treatment groups （P〈0.05）. The number of peripheral blood CD3^＋, CD4^＋T cells and CD4^＋/CD8^＋ were all decreased in oxaliplatin group and combination group, while the num- ber of CDS＋T cells was decreased （P〈0.05）. Compared with nimesulide group, cancer size, cancer weight, integral absorbance of cancer, positive vessel density, the number of peripheral blood CD3^＋, CD4^＋T cells and CD4^＋/CD8^＋ were all decreased in oxaliplat- in group and combination group, while tumor inhibition rate and the number of CD8^＋T cells were all increased （P〈0.05） ; combi- nation group was more significant than oxaliplatin group （P〈0.05）. CONCLUSIONS： Oxaliplatin combined with nimesulide can effectively inhibit cancer growth of esophageal cancer, the mechanism of which may be associated with inhibiting angiogenesis of tumor tissue and strengthening immune function.

关 键 词：食管癌 大鼠 尼美舒利 奥沙利铂 微血管密度 免疫功能 

分 类 号：R965[医药卫生—药理学]