外源性脱落酸对玄参幼苗耐盐碱能力的影响研究  

Study on the Effects of Exogenous Abscisic Acid on Salt-alkali Tolerance of Scrophularia ningpoensis Seedlings

作　　者：王建安[1] 付英杰[1] 王荣华[2] 李艳芝[1] 王欣[1] WANG Jian＇an FU Yingjie WANG Ronghua LI Yanzhi WANG Xin(College of Pharmaceutical Science, Jining Medical University, Shandong Rizhao 276826, China Rizhao Polytechnic, Shandong Rizhao 276826, China)

机构地区：[1]济宁医学院药学院,山东日照276826 [2]日照职业技术学院,山东日照276826

出　　处：《中国药房》2017年第4期573-576,共4页China Pharmacy

基　　金：山东省医药卫生科技发展计划项目(No2013WS0333);济宁医学院重点课题(No.JYZ013KJ007)

摘　　要：目的:研究外源性脱落酸(ABA)对玄参幼苗耐盐碱能力的影响,为在盐碱地种植玄参提供理论依据。方法:50株玄参幼苗分为对照组、盐碱组(75 mmol/L)和盐碱(75 mmol/L)+ABA低、中、高浓度组(10、50、100μmol/L),每组10株。移栽20 d起,每4 d干预1次,2周后测定各组植株生长指标(茎高、鲜质量、干质量、存活率)、生理指标[叶绿素、可溶性糖(SS)、可溶性蛋白(SP)和游离脯氨酸(Pro)含量]、丙二醛(MDA)和过氧化氢(H_2O_2)含量、抗氧化酶活性[超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)活性]、茎和根中Na^+和K^+含量。结果:与对照组比较,盐碱组玄参幼苗茎高、存活率、SP、茎中Na^+和K^+、根中Na^+含量和CAT、GR活性显著降低,叶绿素、Pro、MDA、H_2O_2、根中K^+含量和SOD、POD活性显著升高(P<0.05或P<0.01)。与盐碱组比较,盐碱+ABA中浓度组玄参幼苗茎高、鲜质量、叶绿素含量显著增加,MDA、茎中Na^+含量显著降低;低、中浓度组干质量、SS、SP含量和SOD、CAT活性,高浓度组SS含量显著增加;高浓度组茎高、干质量显著降低;低、高浓度组Pro含量显著降低;低、中、高浓度组H_2O_2、根中Na+含量显著降低,GR活性、茎和根中K^+含量显著升高;中、高浓度组POD活性显著降低。结论:施加一定浓度的外源性ABA可有效提高玄参幼苗耐盐碱的能力,增强植株对盐碱环境的适应能力。OBJECTIVE： To study the effects of exogenous abscisic acid （ABA） on salt-alkali tolerance of Scrophularia ning- poensis seedlings, and to provide theoretic evidence for cultivating S. ningpoensis in salt-alkali soil. METHODS： 50 S. ningpoensis seedlings were divided into control group, salt-alkali group （75 mmol/L）, salt-alkali （75 mmol/L）＋ABA low-concentration, medi- um-concentration and high-concentration groups （10, 50, 100 μmol/L）, with 10 plants in each group. 20 days after transplanting, the plants were sprayed with drugs every 4 days. Growth indexes of the plants （length of stem, fresh weight, dry weight, survival rate）, physiological indexes [the contents of chlorophyll, soluble sugar （SS）, soluble protein （SP） and free proline （Pro）], the contents of MDA and H2O2, the activities of antioxidase [the activities of SOD, POD, CAT, GR] and the contents of Na^＋ and K^＋ in stem and root were determined in each group 2 weeks later. RESULTS： Compared with control group, stem height and survival rate of S. ningpoensis seedlings, SP, the contents of Na^＋ and K^＋ in the stem, the contents of Na^＋, CAT and GR in the roots were all decreased significantly in salt-alkali group; while chlorophyll, Pro, MDA and H2O2 contents, the content of K^＋ in the roots and the activities of SOD and POD were all increased significantly （P〈0.05 or P〈0.01）. Compared with salt-alkali group, stem height of S. ningpoensis seedlings, fresh weight and chlorophyll content were increased significantly in salt-alkali＋ABA medium-concentra- tion group, while MDA and the content of Na^＋ in the stem were decreased significantly; dry weight, the contents of SS and SP, the activities of SOD and CAT were increased significantly in salt-alkali＋ABA low-concentration and medium-concentration groups, while the SS content was increased in salt-alkali＋ABA high-concentration group; stem height and dry weight were decreased signifi- cantly in salt-alkali ＋ ABA high-concentration group; Pr

关 键 词：玄参 盐碱胁迫 外源性脱落酸 生长指标 抗氧化酶 

分 类 号：R931.2[医药卫生—生药学] Q945.78[医药卫生—药学]