光谱法研究人胞红蛋白与铜(Ⅱ)离子的相互作用（英文）  

Spectroscopic Study on the Interaction of Human Cytoglobin with Copper(Ⅱ)Ion

作　　者：李景红[1,2] 边琳[2] 田素燕[2] 孔金明[3] 李连之[2] LI Jing-hong BIAN Lin TIAN Su-yan KONG Jin-ming LI Lian-zhi(Hebei Key Laboratory of Applied Chemistry, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China)

机构地区：[1]燕山大学环境与化学工程学院,河北秦皇岛066004 [2]聊城大学化学化工学院,山东聊城252059 [3]南京理工大学环境与生物工程学院,江苏南京210094

出　　处：《光谱学与光谱分析》2017年第1期321-326,共6页Spectroscopy and Spectral Analysis

基　　金：the National Natural Science Foundation of China(21142003,20471025);the Natural Science Foundation of Shan Province of China(ZR2011BL002)

摘　　要：胞红蛋白(Cygb)是近期在脊椎动物中发现的一种球蛋白家族成员,具有典型珠蛋白的"3+3"式的α-螺旋三明治折叠结构。利用紫外可见吸收光谱、荧光光谱、同步荧光光谱及圆二色(CD)光谱法研究了Cu^(2+)离子与Cygb的相互作用。结果表明,当Cu^(2+)离子加入到Cygb溶液中后,Cygb在280nm处的紫外吸收强度增大,说明Cu^(2+)与Cygb发生了相互作用;Cu^(2+)使Cygb的内源性荧光发生猝灭,其猝灭方式为静态猝灭。同步荧光光谱研究表明,Cu^(2+)可使色氨酸和酪氨酸的微环境发生较小的改变,与酪氨酸相比Cu^(2+)对Cygb的键合部位更接近于色氨酸。圆二色光谱研究表明,Cu^(2+)对Cygb的二级结构未引起明显变化。Cytoglobin（Cygb）,a recently discovered member of the vertebrate globin family,exhibits a traditional globin fold with a three-over-threeα-helical sandwich.The interaction between copper（Ⅱ）ion（Cu2＋）and Cygb has been investigated by UV-Vis,fluorescence,synchronous fluorescence and circular dichroism（CD）spectra.Results showed that the absorption intensity of Cygb at 280 nm increased and the intrinsic fluorescence of Cygb was quenched when Cu2＋was added.This fluorescence quenching of Cygb has been proven that it belongs to static quenching.The synchronous fluorescence spectra indicated that there were small changes about the microenvironment of tryptophan residues and tyrosine residues;furthermore,the binding site of Cu2＋is closer to tryptophan residues than tyrosine residues.No obvious change was observed about the secondary structure of Cygb with the addition of Cu2＋from the CD spectra.

关 键 词：胞红蛋白 铜(Ⅱ)离子 荧光光谱 荧光猝灭 

分 类 号：O629.73[理学—有机化学]