NAA和IBA处理对罗汉松插条生长及生理生化的影响  

Effects of NAA and IBA on Cutting Growth and Physiological and Biochemical Indexes of Podocarpus macrophyllus

作　　者：周启贵[1,2] 陈锡娟 汤春雁 樊春艳[1,2] ZHOU Qi-gui CHEN Xi-juan TANG Chun-yan FAN Chun-yan(School of Life Science, Southwest University, Chongqing 400715, China Key Laboratory of Eco-Environments in Three Gorges Reservoir Region （ Ministry of Education）, Chongqing 400715, China)

机构地区：[1]西南大学生命科学学院,重庆400715 [2]三峡库区生态环境教育部重点实验室,重庆400715

出　　处：《西南大学学报（自然科学版）》2017年第2期28-33,共6页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金项目(31171620);三峡库区生态环境教育部重点实验室自由探索基金项目

摘　　要：以罗汉松扦插条为实验材料,研究了水培条件下,不同质量浓度的生长调节剂吲哚丁酸(IBA)和萘乙酸(NAA)处理对其株质量、株高、生根数等形态指标及多酚氧化酶(PPO)活性、过氧化物氧化酶(POD)活性、叶绿素质量分数、根系活力等生理指标的影响.结果表明:与对照相比,不同质量浓度的IBA和NAA处理均可促进罗汉松水培插条生长,其中质量浓度为60 mg/L IBA及100 mg/L NAA促进株质量、株高效果较好,60 mg/L IBA,60mg/L NAA处理促生根效果最佳;不同质量浓度的IBA,NAA处理中,60 mg/L IBA,100 mg/L NAA的PPO活性和60mg/L IBA,100mg/L NAA的POD活性最高;40mg/L IBA和40mg/L NAA处理组中叶绿素质量分数最高;根系活力与对照组相比差异无统计学意义.因此,不同质量浓度生长调节剂的处理能有效地促进罗汉松的生长并提高PPO,POD活性及叶绿素的质量分数.Hydroponic cuttings of Podocarpus macrophyllus were treated with indolebutyric acid（IBA）and nathphylacetic acid（NAA）at 0,20,40,60,80 and 100mg/L for 2hand then cultivated in the nutrition solution.Determinations were made at intervals of the morphological and physiological-biochemical indexes,including plant fresh weight,plant height,blade number,root number,peroxidase（POD）and polyphenol oxidase（PPO）activities,root activity and the contents of chlorophyll a and b.The results showed that IBA and NAA of different concentrations promoted the weight,height and root growth of the hydroponic P.macrophyllus cuttings.The concentration of 100 mg/L NAA and 60 mg/L IBA gave the best results of promoting plant weight and height,and the hydroponic root growth in the 60mg/L IBA treatment and the60g/L NAA treatment was more significant.After the cuttings of P.macrophyllus were treated with IBA and NAA,the activities of POD and PPO increased regularly.The differences in enzyme activities at the rooting stage were significant.It was indicated that the physiological-biochemical indexes（POD and PPO activity）increased;POD activity was the highest in the 60 mg/L IBA,and 100 mg/L NAA treatments,and PPO activity was the highest in the treatment of 60 mg/L IBA and 100 mg/L NAA.Chlorophyll contents were the highest in the treatments of 40 mg/L IBA and 40 mg/L NAA.Root activity increased by IBA and NAA in different treatments,but was non-significantly different from that of the control.It is concluded that treatments of plant growth regulators at various concentrations promoted the growth of P.macrophyllus cuttings and raised their POD and PPO activities and chlorophyll content.

关 键 词：罗汉松 生长调节剂 生理生化 生长 插条 

分 类 号：Q945.3[生物学—植物学]