4种运动三联征模型大鼠饮食紊乱、物质代谢调控机制比较研究  

Comparison Study on Regulation Mechanism of Eating disorder and Material Metabolism in Four Kinds Model Rats with FAT

作　　者：荆纯祥[1] 赵自明 赖秋媛[1] 汶希[1] 焦润艺[1] 潘华山[1] JING Chunxiang ZHAO Ziming LAI Qiuyuan WEN Xi JIAO Runyi PAN Huashan

机构地区：[1]广州中医药大学,广东广州510006 [2]广东省中医药工程技术研究院,广东广州510095

出　　处：《新中医》2017年第1期228-231,共4页New Chinese Medicine

基　　金：广东省体育局科研项目(GDSS2014204)

摘　　要：目的:探讨持续递增负荷跑台运动和游泳力竭法复制运动三联症(FAT)大鼠模型,及加用放血+环磷酰胺腹腔注射法复制气血亏虚证FAT大鼠模型饮食紊乱与物质代谢紊乱的调控机制。方法:SD大鼠随机分为空白组(正常饮食)、模型1组(持续递增负荷跑台运动+限制饮食)、模型2组(持续递增负荷跑台运动+限制饮食+放血+环磷酰胺)、模型3组(持续递增力竭游泳+限制饮食)、模型4组(持续递增力竭游泳+限制饮食+放血+环磷酰胺)5组,检测造模前后大鼠体质量及8周后大鼠血浆三碘甲状腺原氨酸(T3)、甲状腺素(T4)、促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、皮质酮(CORT)和下丘脑食欲素A含量。结果:与空白组比较,模型1~4组大鼠T3含量和CRH含量变化不明显,差异无统计学意义(P>0.05);模型1~4组大鼠造模后体质量较低,T4含量显著性减少,食欲素A含量显著性降低,ACTH含量显著性增加,差异均有统计学意义(P<0.05,P<0.01)。与模型1组比较,模型2组大鼠造模后体质量、ACTH含量、T4含量、CORT含量均较低,差异均有统计学意义(P<0.05,P<0.01);模型3组大鼠T4含量和CORT含量均较低,差异均有统计学意义(P<0.01,P<0.05)。与模型3组比较,模型4组大鼠造模后体质量、T4含量、ACTH含量、CORT含量均较低,差异均有统计学意义(P<0.01)。结论:持续递增负荷跑台运动和游泳力竭法均可复制FAT大鼠模型,大鼠体质量增加缓慢、下丘脑-垂体-甲状腺(HPT)轴功能降低和下丘脑-垂体-肾上腺皮质(HPA)轴功能亢进;与递增负荷训练模型FAT大鼠比较,气血亏虚证FAT模型大鼠体质量增加更小、HPT轴功能更低而HPA功能亢进不明显。Objective：To discuss regulation mechanism of eating disorder and material metabolism on copying model rats with Female athlete triad（FAT） by gradually increased treadmill exercise and swimming exhausting energy method,and copying model rats with Qi and blood deficiency syndrome merged FAT by bloodletting merged cyclophosphamide peritoneal injection.Methods：Selected SD rats into blank group（normal diet）,model group 1（gradually increased treadmill exercise＋ diet limitation）,model group 2（gradually increased treadmill exercise＋ diet limitation＋ bloodletting＋ cyclophosphamide）,model group 3（gradually increased exhausting swimming＋ diet limitation）,model group 4（gradually increased exhausting swimming＋ diet limitation＋ bloodletting＋ cyclophosphamide）.Detected body mass of rats before and after modeling,and plasma triiodothyronine（13）,thyroxine（T4）,corticotropin-releasing hormone（CRH）,adrenocorticotropic hormone（ACTH）,corticosterone（CORT）,hypothalamus orexin A of rats after 8 weeks.Results：Compared with the blank group,content change of T3 and CRH was not obvious from model group 1 to group 4,there being no significant difference（P〈0.05）;After modeling,body mass of rats were decreased,content of T4 and orexin A were obviously decreased from model group 1 to group 4,ACTH content was obviously increased,the difference being significant（P〈0.05,P〈0.01）.Compared with the model group 1,body mass,content of ACTH,T4 and CORTwere decreased in model group 2 after modeling（P〈0.05,P〈0.01）;Content of T4 and CORT in model group 3 were lower（P〈0.01,P〈0.05）.Compared with model group 3,body mass,content of ACTH and CORT in model group 4 were lower（P〈0.01）.Conclusion：Model rat with FAT can be copied by gradually increased treadmill exercise and swimming exhausting energy method,body mass of rat increase slowly,hyperfunction of hypothalamus-pituitary-thyroid（HPT）axis appear.Compared with model rats with FAT and gradually increased

关 键 词：运动员三联征 饮食紊乱 气血亏虚证 下丘脑-垂体-甲状腺轴(HPT) 下丘脑-垂体-肾上腺皮质轴(HPA) 动物实验 大鼠 

分 类 号：R285[医药卫生—中药学]