Electroacupuncture Intervention Combined with Rat Nerve Growth Factor on Expression of Nestin and NeuN in MCAO Rats  

作　　者：谭峰 陈晶 王健 顾勇 谭玖清 詹杰 程南方 顾明华 粱艳桂 Tan Feng Chen Jing Wang dian Gu Yong Tan diuqing Zhan Jie Cheng Nanfang Gu Minghua Liang Yangui(Foshan Hospital of Traditional Chinese Medicine, Foshan, Guangdong, 528000 Yunnan Provincial Hospital of Traditional Chinese Medicine, Kunming, Yunnan, 650000 Nanfang Hospital Affiliated to Southern Medical University, Guang zhou, Guangdong, 510000)

机构地区：[1]Foshan Hospital of Traditional Chinese Medicine,Foshan,Guangdong,528000 [2]Yunnan Provincial Hospital of Traditional Chinese Medicine,Kunming,Yunnan,650000 [3]Nanfang Hospital Affiliated to Southern Medical University,Guang zhou,Guangdong,510000

出　　处：《新中医》2017年第1期232-237,共6页New Chinese Medicine

基　　金：supported by the National Natural Science Foundation of China(no.81072947,81473470);Natural Science Foundation of Guangdong Province(8152800007000001,2014A030311033,2016A030310239)

摘　　要：Objective:To observe the effect of electroacupuncture(EA) on the expression of Nestin and Neuron specific nuclear protein antigen(neuron-specific protein,NeuN) at different time points after cerebral ischemia reperfusion injury.Methods:One hundred and fifty SD male rats were used to generate a one-side middle cerebral artery occlusion(MCAO) model using thread embolism method,then randomly divided them into the sham operation group,MCAO group,EA group,Nerve Growth Factor(NGF) group and EA+NGF group.The three benzene chloride tetrazolium(TTC) staining was used to detect the volume of cerebral infarction in the rats.Immunohistochemical staining was used to observe the expression of immune positive cell markers such as endogenous neural stem cell(NSC) marker protein Nestin,bromodeoxyuridine(Brdu) and NeuN on 1,7 and 14 days after cerebral ischemia in rats,and compared with the sham operation group.Results:TTC detection of the cerebral infarction volume showed that the nerve function injury score of the EA+ NGF group was significantly lower than MCAO group(P < 0.01).Ischemia led to severe loss of brain function,and EA+ NGF helped in the recovery of cerebral ischemia.Immunohistochemical results showed almost no expression of Nestin in the sham operation group;positive cells was expressed significantly higher Nestin on the 7th day(P < 0.05);then was peaked on the 14 th day.Nestin/BrdU labeled cells in the sham operation group showed minor expression,which was increased on the 7th day(P < 0.05) and was peaked on the 14 th day after ischemia.The differences were statistically significant on the 7th and 14 th day among the EA group,NGF group,EA+NGF group and MCAO group(P< 0.05).NeuN expression was higher in the sham operation group,and was increased on the 7th day after ischemia.NeuN/BrdU labeled cells showed higher expression in the sham operation group,the most obvious improvement was in the EA group,and the NGF and EA+NGF groups also showed significantly increased expression,while MCAO group showed the least.Conclusion:Objective：To observe the effect of electroacupuncture（EA） on the expression of Nestin and Neuron specific nuclear protein antigen（neuron-specific protein,NeuN） at different time points after cerebral ischemia reperfusion injury.Methods：One hundred and fifty SD male rats were used to generate a one-side middle cerebral artery occlusion（MCAO） model using thread embolism method,then randomly divided them into the sham operation group,MCAO group,EA group,Nerve Growth Factor（NGF） group and EA＋NGF group.The three benzene chloride tetrazolium（TTC） staining was used to detect the volume of cerebral infarction in the rats.Immunohistochemical staining was used to observe the expression of immune positive cell markers such as endogenous neural stem cell（NSC） marker protein Nestin,bromodeoxyuridine（Brdu） and NeuN on 1,7 and 14 days after cerebral ischemia in rats,and compared with the sham operation group.Results：TTC detection of the cerebral infarction volume showed that the nerve function injury score of the EA＋ NGF group was significantly lower than MCAO group（P〈0.01）.Ischemia led to severe loss of brain function,and EA＋ NGF helped in the recovery of cerebral ischemia.Immunohistochemical results showed almost no expression of Nestin in the sham operation group;positive cells was expressed significantly higher Nestin on the 7th day（P〈0.05）;then was peaked on the 14 th day.Nestin/BrdU labeled cells in the sham operation group showed minor expression,which was increased on the 7th day（P〈0.05） and was peaked on the 14 th day after ischemia.The differences were statistically significant on the 7th and 14 th day among the EA group,NGF group,EA＋NGF group and MCAO group（P〈0.05）.NeuN expression was higher in the sham operation group,and was increased on the 7th day after ischemia.NeuN/BrdU labeled cells showed higher expression in the sham operation group,the most obvious improvement was in the EA group,and the NGF and EA＋NGF groups also showed significantly incre

关 键 词：中医 症状 临床治疗 患者 

分 类 号：R2-03[医药卫生—中医学]