类叶升麻苷对STZ侧脑室注射模型小鼠认知行为及LTP的影响  

Effect of acteoside on cognitive behavior and LTP of mice induced by streptozotocin intracerebroventricular injection

作　　者：马丁丁 黄晏[2] 李栋[2] 罗嘉楠 闫明 热娜.卡斯木[1] 

机构地区：[1]新疆医科大学药学院,乌鲁木齐830011 [2]军事医学科学院毒物药物研究所,北京100850 [3]新疆维吾尔自治区维吾尔医药研究所 [4]新疆维吾尔医方剂学实验室,乌鲁木齐830049

出　　处：《新疆医科大学学报》2017年第3期328-333,共6页Journal of Xinjiang Medical University

基　　金：新疆自治区科研机构创新发展专项基金(2015007)

摘　　要：目的研究类叶升麻苷对链尿佐菌素(STZ)侧脑室注射诱导的AD模型小鼠认知行为及突触可塑性的影响。方法将昆明小鼠和BALB/c鼠按体质量随机分为正常组、模型组、阳性对照组(Donepezil组)及类叶升麻苷低、中、高剂量组。除正常组注射PBS外,其余各组均侧脑室注射STZ(30μg/μL,5μL)制备AD模型。然后给予5、15、45mg/kg体质量类叶升麻苷溶液灌胃15d。给药完成后,利用自主活动、新物体识别、水迷宫等行为学实验测试小鼠的认知行为,通过突触长时程增强(LTP)观察类叶升麻苷对突触可塑性的影响。结果与模型组小鼠相比,类叶升麻苷给药组小鼠的认知行为及LTP提高,小鼠自主活动性增加,新物体识别优先指数升高,差异有统计学意义(P<0.05),在O迷宫中开臂区域的时间增加,差异有统计学意义(P<0.05),在水迷宫测试期逃避潜伏期缩短,差异有统计学意义(P<0.01),PS波增幅高于模型组,差异有统计学意义(P<0.05)。结论类叶升麻苷对链尿佐菌素(STZ)诱导的AD模型小鼠认知功能具有明显保护作用,其保护作用机制可能与类叶升麻苷可以提高小鼠海马PP-DG区的LTP和改善模型小鼠突触可塑性有关。Objective To investigate the effect of acteoside on cognitive behavior and synaptie plasticity of mice induced by streptozotocin intracerebroventricular injection. Methods Kunming mice or BALB/c mice were randomly divided into control group, model group, positive control group （Donepezil group）, acte-oside low, medium and high dose groups. Every group was induced by STZ intracerebroventricular（i.c.v.） injection （30 μg/μL, 5 μL） except for control group （PBS）. Then mice were treated by different concentrations （5, 15, 45 mg/kg） of acteoside for 15 ds. The cognitive behavior of mice were detected by locomo- tor activity, novel object recognition, Morris water maze test, the affect of synaptic plasticity was observed by LTP. Results Compared with model group, acteoside could improve the cognitive abilities and LTP, their spontaneous motor activity and the new object recognition priority index increased, and the difference was statistically significant （P〈0.05）. Time in open section was increased in the zero maze test, and the difference was statistically significant （P〈0.05）. Escape latency shortened in the Morris, and the difference was statistically significant （P〈0.01）. PS-wave growth was higher than the model group, and the difference was statistically significant （P〈0.05）. Conclusion Acteoside has significantly protective effects on brain damage of mice induced by streptozotocin （STZ）, and it＇s protective mechanism probably related to increasing LTP in hippocampus of mice PP-DG region and improving synaptic plasticity.

关 键 词：链尿佐菌素(STZ) AD模型 认知行为 突触长时程增强 

分 类 号：R96[医药卫生—药理学]