石榴花芽石蜡切片制作方法的改良  

Improvements of Traditional Paraffin Section for Pomegranate Floral Bud

作　　者：陈利娜[1] 薛辉[1] 李好先[1] 牛娟[1] 张杰[1] 刘贝贝[1] 张富红[1] 赵弟广 曹尚银[1] 

机构地区：[1]中国农业科学院郑州果树研究所,河南郑州450009

出　　处：《安徽农业科学》2017年第2期1-3,16,共4页Journal of Anhui Agricultural Sciences

基　　金：中国农业科学院科技创新工程专项(CAAS-ASTIP-2016-ZFRI-03);国家科技基础性工作专项(2012FY110100)

摘　　要：[目的]摸索适合石榴花芽及胚珠结构观察的石蜡切片方法。[方法]以"突尼斯软籽"石榴花芽及胚珠为试材,在传统石蜡切片方法的基础上,结合石榴花芽鳞片厚、子房多室的特点,对样品处理、软化、透明、浸蜡、染色等重要制片环节进行研究。[结果]不同样品处理方式对比发现,将子房纵切4瓣进行固定,切片效果较好。软化结果表明,纱布包裹花芽浸入水中加热至沸腾30 min,有助于提高制片效果。不同染色方式对比发现,爱氏苏木精染色法更适合石榴胚珠切片观察。[结论]通过优化常规石蜡切片方法,得到了适合石榴花芽及胚珠结构观察的石蜡切片方法,该方法同样适用于与石榴花芽及胚珠结构类似的其他结构。[ Objective ] To grope the suitable parafl3n section method for pomegranate floral bud and ovule. [ Method] The floral buds and o- vules of Tunisiruanzi were used as experimental materials for paraffin sectioning to improve sample treatment. Given the character of pomegran- ate floral with thick bud scales and ovary of multicellular, the process of softening, clearing, waxing and staining were improved. [ Result ] To confirm the optimal fixation methods for different parts of ovary, we compared the different fixation methods and found that the best method is fixing after cutting the ovary into four parts using F. A. A.. Softening results showed that if the floral bud was boiled for 30 minutes without stripping scales, the tissues would be complete. Paraffin section staining was performed with Ehrlich hematoxylin, which was the best material for staining for pomegranate floral bud and ovule. [ Conclusion] A modified method of paraffin sectioning for pomegranate floral bud and ovule was recommended on the bases of the traditional method.

关 键 词：石榴 花芽 胚珠 石蜡切片 

分 类 号：S665.4[农业科学—果树学]