当归颗粒剂与煎剂有效成分及体外抑菌活性比较  

Comparison of Active Compositions and Antibacterial Activities in Vitro between Angelica Granule and Decoction

作　　者：孙鹏[1] 张鑫[1] 贺凯 潘琼 王爽[1] 李金荣[1] 郭亚男[1] 曹思婷[1] 白晓南[1] 何生虎[1] 

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]宁夏回族自治区药品检验所,宁夏银川750001 [3]银川第二人民医院药剂科,宁夏银川750001

出　　处：《动物医学进展》2017年第2期50-54,共5页Progress In Veterinary Medicine

摘　　要：为了比较当归颗粒剂与煎剂在有效成分和药效上的差异,采用薄层色谱法(TLC)鉴定当归颗粒剂;高效液相色谱法(HPLC)测定颗粒剂、煎剂和对照药材中阿魏酸的含量;应用《中药色谱指纹图谱相似度评价》软件对颗粒剂、煎剂进行相似度评价,同时进行体外抑菌试验。结果表明,当归颗粒剂TLC图中存在当归对照药材的特征斑点,与对照药材HPLC特征图谱相似度达95.1%;颗粒剂、煎剂和对照药材中阿魏酸的含量分别为3.95、1.69、2.84mg/g;抑菌结果为金黄色葡萄球菌、沙门菌抑菌圈颗粒剂和煎剂相同,大肠埃希菌、链球菌抑菌圈颗粒剂>煎剂。通过试验发现,当归颗粒剂中有效成分与对照药材相近,体外抑菌结果优于煎剂。To compare differences in active compositions and efficacy between Angelica granules and decoction,the Angelica granules were identified by thin layer chromatography （TLC）, the contents of ferulic acid in Angelica granules, decoction and Angelica sinensis radix were detected by high performance liquid chromatography （HPLC）, the similarities of the three solutions were evaluated by ＂chromatographic fingerprint similarity evaluation＂ software. In addition, the antibacterial tests were made. Angelica granule was characterized in the Angelica sinensis radix by TLC figure. HPLC characteristic spectrum similarity was higher than 95.1%. The amount of ferulic acid was 3.95 mg/g, 1.69 mg/g and 2.84 mg/g in granules, decoction and Angelica sinensis radix respectively. The antibacterial results of granules and decoction showed that there is the same inhibition zone to Staphylococcus aureus and Salmonella sp., to Escherichia coli and Streptococcus sp. inhibition zone. granules decoction. The results found that Angelica granules contain active compositions which were similar to Angelica sinensis radix, and showed more effective inhibition results than decoction.

关 键 词：当归颗粒剂 煎剂 薄层色谱法 高效液相色谱法 指纹图谱 体外抑菌 

分 类 号：S853.75[农业科学—临床兽医学]