素心蜡梅组织培养及其体外植株再生技术  

Tissue Culture and Plantlet Regeneration Technology of Chimonanthus praecox cv. Luteus

作　　者：赵振利[1] 曹艳春[2] 刘荣宁[3] ZHAO Zhenli CAO Yanchun LIU Rongning(College of Forestry, Henan Agricultural University,Zhengzhou, Henan 450002 Department of Environmental Art Engineering, Henan Polytechnic College, Zhengzhou, Henan 450046 College of Agricultural Engineering, Henan Vocational College of Agriculture, Zhongmu, Henan 451450)

机构地区：[1]河南农业大学林学院,河南郑州450002 [2]河南职业技术学院环境艺术工程系,河南郑州450046 [3]河南农业职业学院农业工程学院,河南中牟451450

出　　处：《北方园艺》2017年第3期112-115,共4页Northern Horticulture

基　　金：国家林业公益性行业科研专项资助项目(200904009)

摘　　要：以素心蜡梅幼嫩叶片和茎段为试材,进行了组织培养及其体外植株再生技术的研究,为其快速繁殖及工厂化生产奠定基础。结果表明:素心蜡梅的茎段为适宜的外植体,其愈伤组织、芽和根诱导的最适培养基分别为MS+0.1mg·L^(-1) NAA+2.0mg·L^(-1) 6-BA、MS+0.7mg·L^(-1) NAA+2.0mg·L^(-1) 6-BA和1/2MS+0.1mg·L^(-1) NAA。Young stem segments and leaves of Chimonanthus praecoxcv.Luteus were used as materia1 s.A study was conducted on the technology of tissue culture and in vitro plantlet regeneration to provide references for the quick propagation and factory production of Chimonanthuspraecox cv.Luteus.The results showed that stem segment was the appropriate explant,and its appropriate media for callus induction,shoot induction and rooting induction were MS＋0.1mg·L^-1 NAA＋2.0mg·L^-1 6-BA,MS＋0.7mg·L^-1 NAA＋2.0mg·L^-1 6-BA and 1/2MS＋0.1mg·L^-1 NAA,respectively.

关 键 词：素心蜡梅 组织培养 培养基 植株再生 

分 类 号：S685.99[农业科学—观赏园艺]