我国中山病的血清流行病学调查  

The serological epidemiological investigation of Chuzan disease by micro-neutralization test in China

作　　者：张义爽[1] 王芳[2] 武瑞[1] 于力[2] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [2]中国农业科学院哈尔滨兽医研究所牛羊传染病研究创新团队,黑龙江哈尔滨150069

出　　处：《中国预防兽医学报》2017年第1期1-4,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家公益性(农业)科研专项:重要牛羊虫媒病毒病防控关键技术研究与应用(201303035)

摘　　要：2014年本实验室在广西分离获得一株中山病病毒,表明中山病在我国已经存在。为调查中山病在我国的流行分布状况,本实验采用经典的病毒微量中和试验方法,对我国黑龙江、内蒙古、新疆、山东、江苏、湖北、广西、云南和海南等9个省区的543份牛羊血清进行检测。其中,海南68份牛羊血清样品中阳性样品39份,阳性率高达57.35%;黑龙江的80份样品检测结果均为阴性;其他各省的血清阳性率介于6%~48.65%。结果表明,目前被调查的9个省区除黑龙江省外均存在中山病的流行,南方的感染率较高;对新疆、湖北、广西、云南4个省自治区牛和羊的易感性进行比较,除新疆外,牛的阳性率均比羊高,牛血清阳性率6%~58.5%,羊16.67%~33.33%;对不同季节中山病感染率进行比较,结果显示,秋季(9~11月)感染率(16.67%~43.33%)最高,明显高于春季和夏季。由于采集的血清来自具有代表性的省份,以上研究结果表明,中山病在我国的流行具有广泛性。A Chuzan virus （CHUV） was initially isolated by our laboratory from the sentinel cattle in Guangxi province of China in 2014. To investigate the epidemic distribution of Chuzan disease in China, a total of 543 bovine and goat sera derived from 9 provinces were detected for the neutralizing antibodies against CHUV by micro-neutralization test. The highest positive rate of CHUV was up to 57.35% （39/68） in Hainan province, and the samples from Heilongjiang province were all negative. The positive rates in other provinces were between 6% and 48.65 %. The results indicated that Chuzan disease was epidemic severely in 8 of 9 provinces, and the infection rate in the south part of China was higher. The positive rate of bovine sera （6%-58.5%） was higher than goat sera （16.67%-33.33%） in seven provinces except of Xinjiang province. In addition, the results showed that the infection rate in autumn （September to November） was the highest （16.67%-43.33%）, which was significantly higher than that in spring and summer. Due to the representative of collected sera from 9 provinces, these data of serological epidemiological investigation shows that Chuzan disease prevalence in China is extensive.

关 键 词：中山病 微量病毒中和试验 血清流行病学调查 

分 类 号：S852.65[农业科学—基础兽医学]