复方树莓籽粉对急性放射性损伤作用的研究  

Effect of compound raspberry seed powder on acute radiation injury

作　　者：王祯祯[1] 王海涛[3] 高光凯 陈豪[1] 王斌[4] 

机构地区：[1]青岛大学医学院特种医学教研室,266071 [2]解放军第四0一医院全军临床航海医学中心,青岛266071 [3]青岛大学公共卫生学院职业卫生教研室,266071 [4]青岛大学研究生院

出　　处：《中华放射医学与防护杂志》2017年第1期24-28,共5页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的探究复方树莓籽粉对急性放射性损伤大鼠的防护作用。方法40只Wistar大鼠，采用随机数字表法分为4组：空白对照组、单纯照射组、维生素组、复方树莓籽粉组，每组10只。维生素组和复方树莓籽粉组，给予相应药物连续灌胃7d，第7天灌胃结束1h后单纯照射组、维生素组、复方树莓籽粉组进行8Gy一次性全身X射线照射，建立急性X射线损伤模型，24h后处死4组大鼠，检测各组大鼠白细胞数、血小板数、血浆超氧化物歧化酶（SOD）活力、丙二醛（MDA）含量、谷胱甘肽过氧化物酶（GSH—PX）活力，用单细胞凝胶电泳法测定淋巴细胞DNA损伤。结果与单纯照射组比较，维生素组、复方树莓籽粉组大鼠白细胞和血小板数量、SOD活力、MDA含量、GSH—PX活力升高，差异均有统计学意义（F=14．869、6．376、7．705、3．851、3．134，P〈0．05）；单纯照射组大鼠淋巴细胞DNA损伤情况较其他3组严重（F=5．493，P〈0．05）。结论预防性补充复方树莓籽粉对急性放射性损伤大鼠有较好的防护作用。Objective To study the protective effect of compound raspberry seed powder on acute irradiated rat. Methods Totally forty Wistar rats were randomized divided into four groups： normal control group, radiation group, vitamin group, compound raspberry seed powder group. The drugs were intragastrically administrated for 7 days. On 8th day, the rats in each group except control were irradiated with 8 Gy X-rays. 24 h after irradiation, all rats were sacrificed and its WBC, PLT, superoxide dismutases （SOD）, malonaldehyde （MDA）, glutathione peroxidase （GSH-PX） in serum were detected, the DNA damage in peripheral blood lymphocytes （PBL） was measured with the single cell gel electrophoresis technique. Results Compared with radiation alone group, the quantities of WBC, PLT, the activity of SOD, the content of MDA and the activity of GSH-PX in the vitamin group and compound raspberry seed powder group were significantly improved（ F = 14. 869, 6. 376, 7. 705, 3. 851, 3. 134, P 〈 0.05 ）. The DNA damage in PBL of radiation alone group was more severe than that of other three groups （ F = 5. 493, P 〈 0.05）. Conclusions Raspberry seed powder is effective in radiation protection.

关 键 词：复方树莓籽粉 X射线 放射性损伤 超氧化物歧化酶 谷胱甘肽过氧化 物酶 

分 类 号：R285.5[医药卫生—中药学]