6个猪种FUT1基因的多态性分析  

Polymorphism analysis of FUT1 gene in pigs of six breeds

作　　者：杨青春[1] 侯佳 浦忠得 王泽艺 张旗[1] 李萌[1] 靳玉舒 刘宏[3] 郭晓红[1] 曹果清[1] 李步高[1] 高鹏飞[1] YANG Qingchun HOU Jia PU Zhongde WANG Zeyi ZHANG Qi LI Meng JIN Yushu LIU Hong GUO Xiaohong CAO Guoqing LI Bugao GAO Pengfei(College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China Animal Genetic Breeding Center in Shanxi Province, Taiyuan 030027, China Datong Pig Breeding Farm, Datong 037000, China)

机构地区：[1]山西农业大学动物科技学院,山西太谷030801 [2]山西省畜牧遗传育种中心,山西太原030027 [3]山西省大同市种猪场,山西大同037000

出　　处：《畜牧与兽医》2017年第1期1-5,共5页Animal Husbandry & Veterinary Medicine

基　　金：山西省高等学校科技创新项目(2014129);山西省科技攻关项目(041046;20140311020-5;20130311026-1);山西省农业科技成果转化与推广示范项目(SXNKTG09)

摘　　要：α(1,2)岩藻糖转移酶基因1(α-1,2-Fucosyltransferase gene,FUT1)是可以调节大肠杆菌F18侵染猪小肠、并引起仔猪水肿和腹泻的一个抗性基因。采用PCR-SSCP方法,检测了FUT1基因在大白猪、长白猪、杜洛克、马身猪、山西黑猪、晋汾白猪6个猪种中的多态性。结果表明:在大白猪、长白猪和杜洛克这3个引进品种中,存在A、B、C 3种单倍型,A单倍型的平均分布频率为0.58;而在地方品种马身猪及其培育品种山西黑猪和晋汾白猪中只发现了单倍型A。经测序比对发现B单倍型第5位碱基发生了C→A的转换,第145个碱基处发生了T→C的突变;而单倍型C的第372位碱基A发生缺失。大白和杜洛克2个种群Hardy-Weinberg不平衡,FUT1基因在国外引进猪种大白、长白、杜洛克种群多态性丰富。α（ 1,2） fucosyltransferase gene（ FUT1） is an important candidate gene of piglets to resist the Enterotoxigenic Escherichia coli F18 that could induce diarrhea and edema. The present study was conducted to examine the polymorphism of FUT1 gene in pigs of Duroc,Landrace,Mashen,Shanxihei,Shanxibai pigs using the PCR-SSCP. The results indicated that three types of heliotypes A,B and C were found in Yorkshire,Duroc and Landrace pigs. The average frequency of haplotype A was 0. 58. However,only one heliotype was found in native pig breeds. In addition,two mutations located at 5 bp（ C→A） and 145bp（ T→C） in haplotype B were observed. The haplotype C lost base A at 372 bp site. In Yorkshire and Duroc pigs,FUT1 gene didn＇ t reach to Hardy-Weinberg equilibrium status,while,European pig breeds displayed moderate polymorphism. The results of this study have provided the basic data for the application of FUT1 gene in pig breeding.

关 键 词：猪种 FUT1基因 多态性 PCR-SSCP 

分 类 号：S828[农业科学—畜牧学]