辽西地区猪源与人源链球菌耐药性及相关耐药基因同源性分析  

Resistance and homology analysis of the resistance-related genes in Streptococcus isolated from pig and human in western area of Liaoning province

作　　者：孙佳楠 李欣南 伊会杰 李艳 刘英姿 王宏军 高慎阳 盛鹏飞 蒋月 周铁忠 张利勃 SUN Jianan LI Xinnan YI Huijie LI Yan LIU Yingzi WANG Hongjun GAO Shenyang SHENG Pengfei JIANG Yue ZHOU Tiezhong ZHANG Libo(College of Animal Science and Veterinary Medicine, Jinzhou Medical University, Jinzhou 121001, China Liaoning Province Quality and Safety Testing Center of Veterinary Medicine, Feed and Livestock Products, Shenyang 110116, China Jinzhou Animal Quarantine Station in Liaoning, Jinzhou 121001, China Benxi Animal Quarantine Station in Liaoning, Benxi 117020, China)

机构地区：[1]锦州医科大学畜牧兽医学院,辽宁锦州121001 [2]辽宁省兽药饲料畜产品质量安全检测中心,辽宁沈阳110016 [3]辽宁省锦州市动物检疫站,辽宁锦州121000 [4]辽宁省本溪市动物疫病预防控制中心,辽宁本溪117020

出　　处：《畜牧与兽医》2017年第1期55-60,共6页Animal Husbandry & Veterinary Medicine

基　　金：辽宁省科技厅自然科学基金项目(2013022047);国家自然科学基金(31201951)

摘　　要：采用微量稀释法进行11种兽用药及11种人用抗菌药的药物敏感性检测试验。结果表明:猪源和人源链球菌同时对8种兽用抗菌药和10种人用抗菌药物产生表观耐药性。针对分离菌株进行耐药基因检测,选取共同耐药率较高的3类抗菌药物的9种主要相关基因进行检测,发现猪源与人源链球菌携带共同耐药基因有6种,其中四环素类tet L基因的检出率高达92.39%,多黏菌素类mcr-1基因及大环内酯类erm B基因的检出率分别为79.35%和76.09%。对共同携带的耐药基因进行同源性分析表明,耐药基因tet L和erm B的同源性较高,均达到97%以上,其中tet L基因达到100%。研究结果提示猪场猪源和人源链球菌对多种常用兽用抗菌药及人用抗菌药物产生共同表观耐药性,而其部分相关耐药基因具有高度的同源性,这表明细菌耐药性及其耐药基因可在猪源与人源链球菌中传播。The present project was conducted to investigate 11 kinds of veterinary medicines and 11 kinds of human medicines with antibiotic sensitivity using micro-dilution method. The results showed that pig-derived and human-derived Streptococcus generated common apparent resistance on 8 kinds of veterinary and 10 kinds of human medicines. The results showed that pig-derived and human-derived Streptococcus had 6 kinds of resistance genes jointly by selecting nine kinds of major related genes based on three kinds of antimicrobial drugs,in which the tetracyclines tet L gene detection rate was 92. 39%,the polymyxin class mcr- 1 gene and macrolide erm B gene detection rates were 79. 35%and 76. 09%,respectively. In addition,the analysis on nucleotide homology carried common resistance gene showed that homology of tet L and erm B were high,both reached above 97% and tet L reached 100%. Our results showed also that pig-derived and human-derived Streptococcus were common to produce apparent resistance against many common veterinary and human medicines,partial resistance genes associated with a high degree of homology,indicating that bacterial resistance-related genes might spread between pig-derived and human-derived Streptococcus.

关 键 词：链球菌 猪场 耐药基因 同源性分析 

分 类 号：S852.61[农业科学—基础兽医学]