T7 cDNA噬菌体展示文库筛选法氏囊活性肽BPP-Ⅱ结合蛋白及鉴定  

Screening and identification of the binding proteins with BPP-Ⅱ through T7 cDNA phage display

作　　者：周川杰[1] 宗嫚嫚 梁静泊 周广防 冯秀丽[1] 陈溥言[1] 徐生亮[2] ZHOU Chuanjie ZONG Manman LIANG Jingbol ZHOU Guangfang FENG Xiuli CHEN Puyan XU Shengliang(College of Veterinary Medicine, Nanjing Agriculture University, Nanjing 210095, China Hospital of Nanjing Agriculture University, Nanjing 210095, China)

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]南京农业大学校医院,江苏南京210095

出　　处：《畜牧与兽医》2017年第1期71-74,共4页Animal Husbandry & Veterinary Medicine

基　　金：江苏省自然科学基金(BK20130682);国家自然科学基金(31302067)

摘　　要：法氏囊活性五肽BPP-Ⅱ是近期发现的具有免疫调节功能的活性多肽。为了研究法氏囊活性肽对B细胞的作用机制,采用鸡B淋巴细胞cDNA T7噬菌体文库筛选法氏囊五肽BPP-Ⅱ与鸡B相互作用的受体蛋白。三轮筛选后,富集的噬菌体滴度达1010pfu以上。富集噬菌体克隆的序列分析结果表明,与BPP-Ⅱ相互作用的鸡B淋巴细胞cDNA文库中序列与核受体辅阻遏物2同源。结果说明,法氏囊活性肽BPP-Ⅱ可能参与多种细胞过程,从而产生多种生物学效应。The bursa of Fabricius（ BF） is the acknowledged humoral central immune organ,and plays a key role in B lymphocyte development and differentiation. The bursa-derived peptide BPP-Ⅱ is recently reported a biological active peptide on immune induction. However,the mechanism by which this active molecular plays role in B lymphocyte is little reported. To investigate the mechanism,in this paper,the interacting proteins with BPP-Ⅱ were screened by T7 B cell c DNA phage display library. These results showed that after three screening and sequencing,the titer of enriched phage was up to 1010 pfu,and the interacting gene of BPP-Ⅱ in avian B cell was homologous to nuclear receptor corepressor 2. These results suggested that BPP-Ⅱ participates in various biological functions,which provides some new insight for the biological function and mechanism of BF on immune induction.

关 键 词：法氏囊活性肽 BPP-Ⅱ T7噬菌体文库展示 亲和结合 

分 类 号：S855.3[农业科学—临床兽医学]