猪群口腔液与血液中PRRSV和PCV2感染的病原和抗体消长规律调查  

Investigation on pathogen and antibody dynamics of PRRSV and PCV2 infection in oral fluid and serum of pigs

作　　者：位玉玲 李沛东[1,2] 于少芳 郑佳佳 李龙[1,2] WEI Yuling LI Peidong YU Shaofang ZHENG Jiajia LI Long(College of Animal Science and Technology, Zhejiang A ＆ F University, Lin＇an 311300, China Hangzhou Beta Veterinary Diagnostic Laboratory, Hangzhou 310014, China)

机构地区：[1]浙江农林大学动物科技学院,浙江临安311300 [2]杭州贝尔塔兽医诊断实验室,浙江杭州310014

出　　处：《畜牧与兽医》2017年第1期80-85,共6页Animal Husbandry & Veterinary Medicine

基　　金：国家科技型中小企业技术创新项目(14C26213301473)

摘　　要：从浙江某规模猪场分别采集不同日龄商品猪口腔液与血清样本,应用实时定量PCR和ELISA方法,分别检测2种体液中猪繁殖与呼吸综合征病毒(PRRSV)和猪圆环病毒2型(PCV2)的抗原和抗体水平,并分析其消长规律。结果提示:2种病毒各自的特异性抗体在口腔液和血清中的消长规律基本一致。相比血清,口腔液中的母源抗体存续时间更长(3周),主动免疫后产生特异性抗体的时间则更晚(3周)。口腔液中PRRSV和PCV2的感染动态规律,与血清和口腔液抗体的消长规律也具有很好的相关性,在出现病毒感染高峰后,血清和口腔液特异性抗体转阳,其中血清抗体的转阳反应更为迅速(3周)。本研究为进一步发展猪群口腔液检测系统提供了临床依据。In order to investigate pathogen and antibody dynamics of porcine reproductive and respiratory syndrome virus（ PRRSV） and porcine circovirus type 2（ PCV2） infection,both serum and oral fluid of pigs with the different ages from a large-scale pig farm were collected and tested. Both pathogens showed the similar antibody dynamic pattern in serum and oral fluid samples. Compared to serum samples,the viral specific maternal antibody lasted about 3 weeks longer than that in oral fluid samples; however active immune response antibody appeared 3 weeks later in oral fluid samples. PRRSV and PCV2 infection in oral fluid had a good correlation with thedynamics of antibody in serum and oral fluid samples. After the emergence of the peak of virus infection,the two viral antibody positive conversions occurred in serum and oral fluid samples,and the seroconversion was 3 weeks earlier in serum than that in oral fluid samples. This study provides a basis for development of oral fluid detection system.

关 键 词：猪 口腔液 PRRSV PCV2 抗体 

分 类 号：S855.3[农业科学—临床兽医学]