急性力竭运动大鼠脑皮质运动区组织中caspase-3的表达  

Expression of caspase-3 in the cerebral cortical motor area tissue of rats after acute exhaustive exercise

作　　者：刘猛[1] 

机构地区：[1]新乡市中心医院神经外科,河南新乡453000

出　　处：《新乡医学院学报》2017年第1期22-25,共4页Journal of Xinxiang Medical University

摘　　要：目的观察急性力竭运动大鼠脑皮质运动区组织中caspase-3活性和蛋白表达,探讨不同强度力竭运动对脑皮质运动区细胞凋亡的影响。方法健康雄性Sprague-Dawley大鼠80只随机分为对照组(n=8)、中等强度力竭运动组(n=36)和高强度力竭运动组(n=36)。对照组大鼠不进行力竭运动,中等强度力竭运动组和高强度力竭运动组大鼠建立急性力竭运动模型,分别在力竭运动后6 h和1、4、7 d采集大鼠脑皮质运动区组织,采用荧光分析法检测脑皮质运动区组织中caspase-3活性,Western blot法检测caspase-3蛋白水平,比较3组大鼠脑皮质运动区组织中caspase-3活性和蛋白水平。结果力竭运动后6 h,中等强度力竭运动组和高强度力竭运动组大鼠脑皮质运动区脑组织中caspase-3活性显著高于对照组(P<0.05),高强度力竭运动组大鼠脑皮质运动区脑组织中caspase-3活性显著高于中等强度力竭运动组(P<0.05)。力竭运动后1、4 d,高强度力竭运动组大鼠脑皮质运动区脑组织中caspase-3活性显著高于中等强度力竭运动组和对照组(P<0.05),但中等强度力竭运动组与对照组大鼠脑皮质运动区脑组织中caspase-3活性比较差异无统计学意义(P>0.05)。力竭运动后7 d,3组大鼠脑皮质运动区脑组织中caspase-3活性比较差异均无统计学意义(P>0.05)。中等强度力竭运动组大鼠力竭运动后6 h脑皮质运动区脑组织中caspase-3活性显著高于力竭运动后1、4、7 d时(P<0.05)。高强度力竭运动组大鼠力竭运动后1 d时脑皮质运动区组织中caspase-3活性显著高于力竭运动后6 h和4、7 d时(P<0.05),力竭运动后6 h和4 d时脑皮质运动区脑组织中caspase-3活性显著高于力竭运动后7 d时(P<0.05)。与对照组比较,中等强度力竭运动组大鼠力竭运动后6 h和1、4、7 d时脑组织中caspase-3蛋白表达无显著变化(P>0.05);高强度力竭运动组大鼠力竭运动后脑组织中caspase-3蛋白表达逐渐升高,力�Objective To investigate the effect of exhaustive exercise on the apoptosis of nerve cells through detecting the activity and protein expression of caspase-3 in the the cerebral cortical motor area tissue of rats after acute exhaustive exercise. Methods Eighty healthy male Sprague-Dawley rats were divided into control group（ n = 8）,moderate intensity exhaustive exercise group（ n = 36） and high intensity exhaustive exercise group（ n = 36）. The rats in control group didn＇t accept exhaustive exercise,and the rats in moderate intensity exhaustive exercise group and high intensity exhaustive exercise group were used to establish the acute exhaustive exercise model. The cerebral cortical motor area tissues of rats were collected at the time points of 6 hours,4 and 7 days after exhaustive exercise. Caspase-3 activity in the cerebral cortical motor area tissues was detected by fluorescence analysis,and the level of caspase-3 protein was detected by Western blot method. The activity and protein level of caspase-3 in the cerebral cortical motor area tissues of rats were compared among the three groups. Results Six hours after exhaustive exercise,the activity of caspase-3 in the cerebral cortical motor area tissues of rats in moderate intensity exhaustive exercise group and high intensity exhaustive exercise group was significantly higher than that in control group（ P〈0. 05）,and the activity of caspase-3 in the cerebral cortical motor area tissues of rats in high intensity exhaustive exercise group was significantly higher than that in moderate intensity exhaustive exercise group（ P〈0. 05）. One and four days after exhaustive exercise,the activity of caspase-3 in the cerebral cortical motor area tissues of rats in high intensity exhaustive exercise group was significantly higher than that in moderate intensity exhaustive exercise group and control group（ P〈0. 05）,but there was no significant difference in the activity of caspase-3 between moderate intensity exhaustive exercise group and contro

关 键 词：CASPASE-3 力竭运动 皮质运动区 细胞凋亡 

分 类 号：R874[医药卫生—运动医学]