c-kit与miR-21在心衰大鼠心肌中表达下降  

Decreased c-kit and miR-21 gene expression in rat heart failure

作　　者：刘振[1] 李硕[1] 刘玲玲[1] 郭志坤[2] 王鹏飞[1] 

机构地区：[1]新乡市中心医院心血管内科,河南新乡453003 [2]新乡医学院河南省医用组织再生重点实验室,河南新乡453003

出　　处：《基础医学与临床》2017年第1期76-79,共4页Basic and Clinical Medicine

摘　　要：目的研究c-kit与miR-21基因在大鼠左室重构中的动态表达及其作用机制。方法将大鼠140只随机分为正常对照组15只和心衰模型组125只。心衰模型制作:4 mg/kg腹腔注射阿霉素。在8周时对大鼠进行心功能检测,验证心衰模型。取心脏组织冰冻切片。利用免疫组化及免疫荧光显色等技术检测心肌组织中miR-21和c-kit的基因表达。结果 1)心衰组的呈现心肌梗死后心肌细胞的病理学变化。2)在正常和心衰心肌中miR-21阳性细胞主要表达于血管内皮,少量心肌细胞和干细胞,而心衰心肌组织中的表达量明显减少;c-kit阳性细胞常成群分布,主要聚集于心外膜及其附近。在两组心肌组织中均有少数细胞存在miR-21和c-kit共表达。结论 c-kit和miR-21在心衰大鼠心肌中表达下降与心力衰竭和左室重构呈高度相关。Objective To explore mechanism（ s） is dynamic expression and internal relations of c-kit gene and miR-21 in rat ventricular remodeling. Methods SD rats（ n = 140） were randomly divided into normal control group（ n = 15） and heart failure model group（ n = 125）. Heart failure model： 4 mg /kg intraperitoneal injection of adriamycin. To detect the cardiac function of rats in eight weeks,proving the heart failure. Then harvest the rat heart,frozen section,using immunohistochemistry and immunofluorescence color to detect the expression changes of miR-21 and c-kit in myocardial tissue. Results The emergence of the pathological changes of cardiac muscle cells after myocardial infarction in the heart failure groups and the control group didn＇t appear the myocardial infarction and heart failure. Immunohistochemistry and immunofluorescence show that miR-21 positive cells in normal and heart failure myocardium mainly express in vascular endothelium,a few myocardial cells and stem cells,and endocardial expressing quantity fell more than epicardial; c-kit positive cells tend to cluster together,mainly gather in the epicardial and its nearby,and the expressing quantity decrease significantly after heart failure. A small number of cells exsit miR-21 and c-kit common expression in both groups（ P〈 0. 05）. Conclusions The decreased expression of c-kit and miR-21 is highly related to rats heart failure and left ventricular remodeling.

关 键 词：C-KIT MIR-21 免疫组织化学 免疫荧光 心力衰竭 大鼠心脏 

分 类 号：R541.6[医药卫生—心血管疾病]