高糖及高胰岛素对人血管平滑肌细胞增殖、迁移及miR-145水平的影响  

Effects of both high glucose and high insulin on proliferations and migrations of human vascular smooth muscle cells as well as miR-145 level

作　　者：金成吉[1] 唐田[2] 于晓静[1] 曲建梅[1] 段薇[1] 张弢[1] 

机构地区：[1]大连大学附属中山医院内分泌科,辽宁大连116001 [2]聊城市人民医院内分泌科,山东聊城252000

出　　处：《基础医学与临床》2017年第1期94-97,共4页Basic and Clinical Medicine

摘　　要：目的探讨高血糖和高胰岛素对人miR-145水平的影响。方法将人血管平滑肌细胞(VSMC)分为正常组、高糖组(25 mmol/L)、高胰岛素组(300 m U/L)和高糖高胰岛素组,各组细胞培养72 h后,用real-time PCR测定各组VSMC中miR-145的水平;用四甲基偶氮唑盐(MTT)法及细胞划痕法分别测定VSMC的增殖及迁移。miR-145病毒转染VSMC 48 h后,再分为上述4组,用上述方法测定病毒转染后VSMC的增殖与迁移。结果与正常组相比,其他3组miR-145水平均不同程度下降(P<0.05),尤以高糖高胰岛素组下降最明显(P<0.01);高糖及高胰岛素促进VSMC的增殖和迁移。miR-145病毒转染后,各组VSMC的增殖和迁移均较转染前明显降低(P<0.05)。结论高血糖及高胰岛素降低VSMC中miR-145的表达,miR-145的过表达可抑制VSMC的增殖和迁移。Objective To identify the effect of both high glucose and high insulin on miR-145 level. Methods The human vascular smooth muscle cells（ VSMCs） were cultured and the proliferation of VSMCs was induced by high glucose and high insulin medium. The samples were divided into 4 groups： normal group,high glucose group（ 25 mmol / L）,high insulin group（ 300 m U / L）,high glucose and high insulin group. The expression of miR-145 in VSMCs was assayed by real-time PCR. Proliferation of VSMCs was determined by MTT method After 72 h cultivation. The migration of VSMCs was analyzed by cell scratch test. VSMCs in each group was transfected by miR-145 virus（ lentiviral vector）. Proliferation and migration were assayed after48 h transfection. Results The expression of miR-145 in VSMCs of other three groups was decreased（ P 〈0. 05）,especially the expression in the high glucose and insulin group was the lowest（ P〈 0. 01）. Proliferation and migration of VSMCs was promoted by high glucose and / or high insulin medium. Under fluorescent,transfection rate of VSMCs was about 80 % after 48 h transfection. Proliferation and migration of VSMCs in eachgroup after transfection were significantly lower than before（ P〈 0. 05）. Conclusions High glucose and high insulin could decrease the expression of miR-145 in VSMCs,The overexpression of miR-145 may inhibit the proliferation and migration of VSMCs.

关 键 词：MIR-145 血管平滑肌细胞 糖尿病 

分 类 号：R587.2[医药卫生—内分泌] R54[医药卫生—内科学]