大黄酸对3T3-L1前脂肪细胞增殖分化及相关基因表达的影响  

Effect of Rhein on 3T3-L1 Preadipocyte Differentiation and Related Gene Expression

作　　者：李佳佳[1] 梁耀月 董世芬[1] 杨蓉芳 肖丛瑞 王晶[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中医药信息》2017年第1期1-5,共5页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81503287;81430094);教育部博士点基金项目(No.20130013120002)

摘　　要：目的:研究大黄酸(1,8-dihydroxy-3-carboxyanthraquinone,Rhein,Rh)对3T3-L1前脂肪细胞增殖分化及相关基因表达的影响,从分子生物学角度探讨大黄酸降脂的作用机理。方法:体外培养3T3-L1前脂肪细胞,采用噻唑蓝法(MTT)研究大黄酸(5、10、20、40、80、160μM)对3T3-L1前脂肪细胞增殖活性的影响;大黄酸(20、40、80μM)干预3T3-L1脂肪细胞诱导分化,油红O染色分析大黄酸对3T3-L1脂肪细胞分化过程中胞浆脂滴积累的影响;生化法检测大黄酸对3T3-L1脂肪细胞中甘油三酯(Triglyceride,TG)的影响;实时荧光PCR法检测过氧化物酶体增殖物激活受体γ(Peroxisome Proliferator Activated Receptor Gamma,PPARγ)、CCAAT增强子结合蛋白(CCAAT/enhancer binding protein",C/EBPα)、脂肪酸合成酶(Fatty acid synthetase,FAS)mRNA的表达水平。结果:大黄酸160μM对3T3-L1前脂肪的增殖有显著的抑制作用,大黄酸20、40、80μM剂量依赖地降低3T3-L1脂肪细胞中脂滴的积累,同时显著降低3T3-L1脂肪细胞中TG含量,并且下调3T3细胞中脂肪分化相关基因PPARγ、C/EBPα、FAS mRNA的表达。结论:大黄酸能够抑制3T3-L1脂肪细胞的诱导分化,降低细胞内TG含量,其作用机制可能与下调脂肪分化相关基因PPARγ、C/EBPα、FAS mRNA的表达有关。Objective ： The effect of rhein （ 1,8 - dihydroxy - 3 - carboxyanthraquinone, Rhein, Rh） on the proliferation and differentiation of 3T3 - L1 adipocyte and the related gene mRNA expression was investigated. Method ：MTT assay was used to detect 3T3 - L1 cell proliferation by rhein （5,10,20,40,80,160 μM）, rhein intervention 3T3 - L1 adipocytes induced differentiation, oil red 0 staining and colorimetric analysis of the impact of rhein on 3T3 - L1 adipocyte differentiation process of the accumulation of lipid droplets in the cyto- plasm, biochemical detected rhein 3T3 - L1 adipocytes triglyceride （TG）. The expression of CCAAT enhan- cer binding proteins （C/EBPα）, fatty acid synthetase （FAS）, peroxisome proliferators activated receptor γ （PPARγ） genes was measured by real - time PCR. Results ： The results showed that rhein （20,40,80 μM） could inhibit adipocyte differentiation and reduce intracellular TG content in a dose - dependent manner. Furthermore, rhein down- regulated the mRNA expression of C/EBPα, FAS, and PPAR~/ genes. Conclusion： Rhein can inhibit the differentiation of 3T3 -L1 adipocytes, and the mechanism of action may be related to reducing adipose differentiation -related gene PPART, C/EBPα and FAS expression.

关 键 词：大黄酸 3T3-L1前脂肪细胞 增殖分化 基因表达 

分 类 号：R285[医药卫生—中药学]