附子半夏汤对阳虚模型大鼠不同脏器能量代谢的影响  

Effect of Fuzi Banxia Tang on the Energy Metabolism of Different Organs of Yang Deficiency Model Rats

作　　者：滕培颍 赵瑞芝[1] 徐福平[1] 赵亚[1] 杨志敏[1] 

机构地区：[1]广州中医药大学第二临床医学院,广东广州510120

出　　处：《新中医》2017年第2期4-6,共3页New Chinese Medicine

基　　金：国家自然科学基金资助项目(81503515);广东省自然科学基金资助项目(2015A030310255);中国博士后科学基金资助项目(2015M572295);广东省财政厅项目(2013KT1498)

摘　　要：目的:观察阳虚模型大鼠不同脏器能量代谢水平及附子半夏汤对阳虚模型大鼠不同脏器能量代谢的影响。方法:SD大鼠30只,随机分为3组,分别为正常组、模型组、中药组,除正常组外,其他2组采用肌注氢化可的松注射液,同时灌胃大黄水煎液复制阳虚大鼠模型。造模17天后,中药组灌胃附子半夏汤水煎液2周,给药结束后分别检测各组大鼠心、肝、脾、肾、小肠组织Na^+-K^+-ATP酶的活力。结果:造模第17天时,模型组和中药组大鼠出现体毛枯疏无泽、活动减少、精神不振、爪甲冰凉、体质量增长迟缓、肛温下降等表现。与正常组比较,模型组和中药组大鼠第17天和第31天体质量较低,差异均有统计学意义(P<0.01,P<0.05);造模第17天时,模型组和中药组肛温均显著性下降,差异均有统计学意义(P<0.01)。与给药前比较(即第17天),中药组给药后(即第31天)肛温出现显著性上升,差异有统计学意义(P<0.05)。实验结束后,与正常组比较,模型组的肝脏、心脏、肾脏、小肠的Na^+-K^+-ATP酶活性均显著性下降,差异均有统计学意义(P<0.05,P<0.01);与模型组比较,中药组的心脏、肾脏、脾脏、小肠的Na^+-K^+-ATP酶活性增高,差异均有统计学意义(P<0.01)。结论:阳虚模型大鼠不同脏器能量代谢水平均显著下降,附子半夏汤水煎液能显著提高阳虚大鼠心、肾、脾、小肠组织的能量代谢水平。Objective：To observe the energy metabolism level of different organs of Yang deficiency model rats and the effect of Fuzi Banxia tang on the energy metabolism of different organs of Yang deficiency model rats. Methods：Selected 30 SD rats and divided them into three groups randomly,the normal group,the model group and the Chinese medicine（CM） group. Expect the normal group,other two groups were given intramuscular hydrocortisone and at the same time reconstructed Yang deficiency model rats by administering Dihuang water decoction. After modeling,the CM group was administered Fuzi Banxia tang water decoction for 2 weeks.Then,measured the Na~＋-K~＋-ATP enzyme activity of the heart,liver,spleen,kidney and intestinal tissue of the rats in each group respectively. Results：Seventeen days after modeling,the rats in the model group and CM group appeared dry and lackluster hair,less activity,lassitude,cold paws and nails,slow growth of body mass,decrease of rectal temperature and other manifestations. Compared with the normal group,the body mass of the rats in model group and CM group were lower at 17 th day and 31 st day（P〈0.05,P〈0.01）.At the 17 th day,the rectal temperature of the rats in model group and CM group both significantly decreased（P〈0.01）. Compared with that before medication,at the 17 th day,the rectal temperature of the rat in CM group appeared significantly increased after medication at the 31 st day（P〈0.05）. After experiment,compared with the normal group,the Na~＋-K~＋-ATP enzyme activity of the liver,heart,kidney and small intestine of the rats in the model group were all significantly declined（P〈0.05,P〈0.01）. Compared with the model group,the Na~＋-K~＋-ATP enzyme activity of the heart,kidney,spleen and small intestine of the rats in the CM group were significantly increased（P〈0.01）. Conclusion：The energy metabolism level of different organs of Yang deficiency model rats were all significantly decreased.Fuzi Banxia tang water decoction can significant

关 键 词：附子半夏汤 能量代谢 NA^+-K^+-ATP酶活性 阳虚模型 大鼠 动物实验 

分 类 号：R285[医药卫生—中药学]