中成药逆转人结肠癌细胞株对化疗药物耐药的研究  

Research on Chinese Patent Medicine Reversing Chemotherapeutic Drugs Resistance of Human Colon Carcinoma Cell Line

作　　者：张念华[1] 陈高峰[1] 李寿杰[1] 武如通[1] 高海利[1] 

机构地区：[1]广东省第二中医院肿瘤科,广东广州510095

出　　处：《新中医》2017年第2期11-14,共4页New Chinese Medicine

基　　金：广东省中医药局科研课题(20151006)

摘　　要：目的:本研究拟观察鸦胆子油乳注射液、华蟾素注射液和注射用黄芪多糖对结肠癌耐药细胞株的杀伤作用,为临床合理、有效使用中成药提供实验依据。方法:运用药物浓度梯度递增间歇诱导法建立人结肠癌lovo细胞耐5-氟尿嘧啶细胞株,并命名为lovo/5-Fu。MTT法检测lovo/5-Fu的耐药能力及不同浓度中成药对lovo/5-Fu细胞株的耐药逆转作用,并根据相应结果计算耐药逆转倍数(RF)。细胞迁移实验分为4组:空白对照组、黄芪多糖组、鸦胆子组和华蟾素组,检测不同中成药对lovo/5-Fu细胞株迁移能力的影响。结果:5-Fu对lovo细胞株的IC_(50)为14.5 mg/L,对lovo/5-Fu细胞株的IC_(50)为115.1 mg/L,根据上述两株细胞的IC_(50)可算出lovo/5-Fu细胞株对5-Fu的耐药指数为:7.94。浓度为17.3、45.3、83.8、112.5 mg/L鸦胆子油乳注射液的耐药逆转倍数分别为9.5、23.5、54.4、86.5倍;浓度为8.2、21.2、38.6、50.1 mg/L华蟾素注射液的耐药逆转倍数分别为11.2、25.2、56.6、90.5倍;浓度为26.3、70.9、115.2、163.4 mg/L注射用黄芪多糖的耐药逆转倍数分别为1.5、8.2、26.5、74.2倍。与空白对照组比较,黄芪多糖组、鸦胆子组和华蟾素组侵出小室的细胞数目明显减少,迁移能力明显减弱,其中以鸦胆子组和华蟾素组更为明显,差异均有统计学意义(P<0.05)。结论:鸦胆子油乳注射液、华蟾素注射液和注射用黄芪多糖这三种中成药均能不同程度的逆转lovo/5-Fu对5-Fu的耐药性,其中以鸦胆子油乳注射液和华蟾素注射液最为明显。Objective： This study was to observe killing effect of Fructus bruccae emulsion, cinobufagin injection and Astragalus polysaccharides for injection on colon carcinoma drug-resistant cell line, and provide experimental basis for clinical use of Chinese patent medicine properly and effectively. Methods： Establish colon carcinoma lovo cell line which is5-fluorouracil（5-Fu） resistance with the method of drug concentration gradient and increasing intermittent revulsion,and named it lovo/5-Fu. Detected Drug resistance ability of lovo/5-Fu and drug resistance reversal of Chinese patent medicine of different concentration in lovo/5-Fu with methyl thiazolyl tetrazolium（MTT） assay,calculated drug resistance reversal fold（RF） according to the corresponding results. Cell migration experiment divided objects into four groups： blank control group, Astragalus polysaccharides group,khosam group and cinobufagin group,and detected effect of different Chinese patent medicine on transfer ability of lovo/5-Fu cell line. Results： IC50（half maximal inhibitory concentration） of lovo cell line in 5-Fu was 14.5 mg/L,IC50 of lovo/5-Fu lovo cell line in 5-Fu was 115.1 mg/L. According to the above IC50 cell lines,the multidrug resistance index of lovo/5-Fu cell line in 5-Fu was 7.94. Drug resistance RF of Fructus bruccae emulsion was 9.5,23.5, 54.4,86.5,whose concentration was 17.3, 45.3, 83.8, 112.5 mg/L respectively; drug resistance RF of cinobufagin injection was11.2, 25.2, 56.6, 90.5, whose concentration was 11.2, 25.2, 56.6, 90.5 mg/L respectively; drug resistance RF of Astragalus polysaccharides for injection was 1.5,8.2,26.5,74.2,whose concentration was 26.3,70.9,115.2,163.4 mg/L respectively. Compared with the blank control group, cell number that cut out of the small room and transfer ability of Astragalus polysaccharides group, khosam group and cinobufagin group were obviously decreased, which were more significant in the khosam group and cinobufagin group（P 〈0.05）. Conclusion： Fructus bruccae emulsi

关 键 词：人结肠癌lovo细胞株 多药耐药性 5-氟尿嘧啶(5-Fu):lovo/5-Fu细胞株 鸦胆子油乳注射液 华蟾素注射液 注射用黄芪多糖 

分 类 号：R285.5[医药卫生—中药学]