壳聚糖-银纳米微粒表面修饰木纤维的制备及抗菌性能研究  

Preparation and Antibacterial Activity of Wood Fiber Modified with Chitosan-Ag Nanoparticles

作　　者：冯晓燕[1,2,3,4] 郑坤[1,2,3,4] 陈莹[1,2,3,4] 王春鹏[1,2,3,4] 储富祥[1,2,3,4] 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室 [4]江苏省生物质能源与材料重点实验室,江苏南京210042

出　　处：《生物质化学工程》2017年第1期1-7,共7页Biomass Chemical Engineering

基　　金：江苏省生物质能源与材料重点实验室基本科研业务费项目(JSBEM-S-201503);江苏省自然科学基金项目(BK20131071)

摘　　要：以液相还原法制备了壳聚糖-银纳米微粒(CS-Ag NPs),并通过γ-巯丙基三甲氧基硅烷作为硅烷偶联剂将其修饰到木纤维表面,获得了具有抗菌性能的CS-Ag NPs表面修饰木纤维。采用透射电子显微镜研究了CS-Ag NPs的形貌;以傅里叶变换红外光谱、扫描电子显微镜、热重分析仪等分析手段对CS-Ag NPs表面修饰的木纤维进行了结构形貌以及热稳定性的分析,并讨论了其吸水性能。结果表明表面修饰后的木纤维接枝率为3.06%,具有高热稳定性及低吸水性。通过琼脂板计数法测试了CS-Ag NPs表面修饰前后的木纤维对耐甲氧西林金黄色葡萄球菌、革兰氏阳性菌金黄色葡萄球菌、蜡状芽庖杆菌以及革兰氏阴性菌大肠埃希氏菌的抗菌效果,结果显示表面修饰后的木纤维对实验菌种的抑菌率都在99.0%以上。此外,还研究了未处理、PVP-Ag NPs以及CS-Ag NPs表面处理的木纤维板的抗菌性能,结果表明,在湿热环境下,CS-Ag NPs表面处理的木纤维板到第8天时仍没有霉菌长出,而未处理的木纤维板和传统抗菌剂银纳米微粒处理的木纤维板到第5天时就开始长霉菌,由此可以看出表面修饰CS-Ag NPs的木纤维板具有更好的抗菌效果。Chitosan-Ag nanoparticles（ CS-Ag NPs） were prepared by liquid phase reducing method and then modified on the wood fiber with γ-mercaptoproyl trimethoxysilane as coupling agent to obtain the antibacterial wood fiber. The morphology of CS-Ag NPs was analyzed by TEM. The morphology and chemical structure as well as the thermal stability of the modified wood fiber were characterized by FT-IR,SEM and TG. Moreover,the water absorbency of the wood fiber was explored. The results indicated that the modified wood fiber showed high thermal stability and low water absorbency. The antibacterial effects of the wood fiber against methicillin-resistant Staphylococcus aureus（ MRSA）,gram-positive bacteria Staphylococcus aureus,Bacillus cereus and gram-negative bacteria Escherichia coli were studied with AGAR plate counting method. It showed that the inhibitory rates of the modified wood fiber against the bacteria were all up to 99. 0 %. Furthermore,the antimicrobial properties of the wood fiber board treated with PVP-Ag NPs and CS-Ag NPs were investigated. It indicated that the wood fiber board treated with CS-Ag NPs did not find bacterial plaque at 8th day while the fibers treated with PVP-Ag NPs and untreated found the plaque at 5th day in hygrothermal environment.The results illustrated that the wood fiber board treated with CS-Ag NPs possessed greater antibacterial properties.

关 键 词：银纳米微粒 木纤维 抗菌 

分 类 号：TQ351.0[化学工程]