重庆北碚桑树萎缩病植原体分子鉴定  

Molecular Detection of Phytoplasma Associated with Mulberry Dwarf Disease in Beibei of Chongqing

作　　者：赵雪君[1] 张宁[1] 李婷婷[1] 赵城钢 孙现超[1] 

机构地区：[1]西南大学植物保护学院/植物病害生物学重庆市高校级重点实验室,重庆400716

出　　处：《西南大学学报（自然科学版）》2017年第1期16-20,共5页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市自然科学基金项目(CSTC2010BB1122);中央高校基本科研业务费资助项目(XDJK2016A009;2362015xk04)

摘　　要：运用16S rDNA基因分子检测技术,使用16m F2/R16mR1,R16F2n/R16R2引物对扩增采集于重庆北碚地区表现萎缩病症的桑叶样品的总DNA,结果发现在编号为C,I,S的样品中扩增出了约1.2 kb的特异条带.经克隆测序并通过NCBI比对分析后,确定所得序列为植原体的特定序列16S rDNA,将测序结果与已报道的桑树萎缩病植原体序列进行同源性比对,结果表明重庆北碚地区桑树萎缩病植原体均属于16S r I亚组,其中C样品中萎缩病植原体鉴定为B亚组.研究结果为明确北碚地区桑树萎缩病病原及该病害的有效防治提供了基础依据.In order to provide basic reference for the identification of the pathongen of mulberry dwarf disease and for its control,mulberry leaves showing dwarf symptoms were collected from Beibei,Chongqing,and the universal phytoplasma primer pairs R16 mF2/R16mR1 and R16F2 n/R16R2 were used to amplify 16 S rDNA of phytoplasma from each leaf sample.In the samples of No.C,I and S,specific fragments of about 1.2 kb were obtained.After cloning and sequencing,the obtained sequences were identified as the specific fragments of phytoplasma 16 S rDNA.Results of Blast in NCBI showed that the pathogen of mulberry dwarf disease in Beibei belonged to the 16 S r I group of phytoplasma.Phytoplasma in the C sample belonged to subgroup B of 16 S r I.

关 键 词：桑树萎缩病 植原体 16S RDNA基因 多样性 

分 类 号：S432[农业科学—植物病理学]