肉牛溶血性曼氏杆菌的分离鉴定及药敏试验  

Isolation,Identification and Susceptibility Testing of Mannheimia haemolytica in Beef Cattle

作　　者：张升杨 李前勇[1] 张德志[1] 詹飞[1] 别凤斌 

机构地区：[1]西南大学(荣昌校区)动物医学系,重庆荣昌402460

出　　处：《西南大学学报（自然科学版）》2017年第1期21-24,共4页Journal of Southwest University(Natural Science Edition)

基　　金：国家科技支撑计划项目(2011BAD36B01);重庆市科技支撑示范工程项目(jcsf121-2012-01-1)

摘　　要：从发生运输应激病牛鼻咽部采取鼻拭子3个,经细菌分离培养、致病性试验、生化试验及16S rRNA序列分析,结果表明所分离出的3株菌均为致病性、溶血性曼氏杆菌;KB药敏试验法证实,3株菌对磷霉素、菌必治、阿米卡星、克拉霉素、阿奇霉素、乙酰螺旋霉素等呈现出高度敏感,而对牛病临床常用的青霉素类、头孢菌素类、氨基糖苷类药物表现出明显的耐药性.Three nasopharyngeal swabs were taken from sick cattle with transport stress syndromes,and bacterial isolation and culture,pathogenicity test,biochemical test and 16 SrRNA sequence analysis were made.The results showed that the three strains isolated were pathogenic Mannheimia haemolytica.In a KB sensitivity test,the three strains were confirmed to be highly sensitive to Fosfomycin,Ceftriaxone,Amikacin,Clarithromycin,Azithromycin and Acetylspiramycin,and resistant to such drugs commonly used in clinical treatment of the cattle disease as Penicillins,Cephalosporins and Aminoglycosides.

关 键 词：肉牛 溶血性曼氏杆菌 分离鉴定 药敏试验 

分 类 号：S823[农业科学—畜牧学]