鞘内预注布托啡诺与吗啡对炎性痛大鼠痛觉及脊髓c-fos蛋白表达的影响  

Effect of intrathecal preinjection of butorphanol and morphine on the expression of c-fos protein and pain behavior in the spinal cord of rats with inflammatory pain

作　　者：周婧[1] 谈大海[1] 金毅[2] ZHOU jing TAN Dahai JIN Yi(Department of Anesthesia, Hangzhou Cancer Hospital, Hangzhou 310002, China)

机构地区：[1]杭州市肿瘤医院麻醉科,浙江杭州310002 [2]南京军区南京总医院疼痛科

出　　处：《全科医学临床与教育》2017年第1期10-13,共4页Clinical Education of General Practice

摘　　要：目的观察鞘内预先注射布托啡诺与吗啡对福尔马林诱导的炎性痛大鼠疼痛行为学及脊髓c-fos蛋白表达的影响。方法大鼠被随机分为布托啡诺组、吗啡组及对照组,每组8只。布托啡诺组鞘内预先注射布托啡诺10μg,吗啡组鞘内预先注射吗啡10μg,对照组鞘内预先注射0.9%氯化钠注射液,然后在三组大鼠的左后足掌面皮下注射福尔马林致痛,观察各组大鼠行为学变化并通过免疫组化法测定大鼠腰4~6节段脊髓背角c-fos受体的表达。结果与对照组比,吗啡组和布托啡诺组没有明显的福尔马林致痛双相反应,且福尔马林致痛大鼠腰4-6脊髓背角浅层及深层fos样免疫反应神经元(FLIN)数量明显减少(q分别=5.65、4.78;5.85、6.27,P均<0.05),而与吗啡组比较,布托啡诺组大鼠行为学表现更安静,疼痛加权评分减低,脊髓背角浅层及深层FLIN数量也明显减少(q分别=9.53、6.37,P均<0.05)。结论鞘内预先注射布托啡诺与吗啡能够对福尔马林致痛大鼠产生明显的抗伤害和镇痛作用,且布托啡诺的镇痛效果优于吗啡。Objective To observe the effect of intrathecal injection of butorphanol and morphine on pain behavior of rats with inflammatory pain induced by formalin,and compare their analgesic effect. Methods All rats were randomly divided into 3 groups,8 rats in each group： butorphanol group（intrathecal preinjection of butorphanol 10 μg）,morphine group（intrathecal preinjection of morphine 10 μg）and control group（intrathecal preinjection of physiological saline）.After the formalin were injected,the behavior of rats were observed. And the expression of c-fos receptor in the Loinl4~6 spinal dorsal horn in rats were determined by immunohistochemical method. Results Compared with control group,the morphine group and butorphanol group had not obvious pain in the two phase reaction,and the expression of c-fos receptor in the loinl4~6 spinal dorsal horn in rats was significantly lower（q=5.65,4.78;5.85,6.27,P〈0.05）.Compared with morphine group,the behavior of rats in butorphanol group was more quiet,the pain score decreased and c-fos receptor expression was significantly decreased（q=9.53,6.37,P〈0.05）. Conclusion Intrathecal injection of butorphanol and morphine could play obvious anti- injury and analgesic effect on the pain induced by formalin,and the analgesic effect of butorphanol was better than morphine.

关 键 词：布托啡诺 吗啡 福尔马林 c-fos受体 

分 类 号：R614[医药卫生—麻醉学]