白藜芦醇预处理对大鼠皮质神经元氧糖剥夺/再复氧损伤后神经元突起生长的影响  

Effects of resveratrol pretreatment on neurite growth of primary cortical neurons after oxygen-glucose deprivation/reperfusion injury in rats

作　　者：唐凡人 余萍萍[1] 王莉[1] 郭霜[1] 杨琴[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科,重庆400016

出　　处：《解剖学报》2017年第1期1-6,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金(81071119);国家神经病学临床重点专科建设项目[卫办医政函(2012)649号]

摘　　要：目的探讨白藜芦醇预处理对大鼠皮质神经元氧糖剥夺/再复氧损伤后神经元突起生长的影响。方法体外皮质神经元氧糖剥夺150min,复氧培养24 h。实验分为正常组、对照组和5μmol/L白藜芦醇预处理组。免疫荧光法鉴定神经元,细胞计数试剂盒8(CCK-8)法测定细胞活力,TUNEL法检测细胞凋亡,免疫荧光法和Western blotting检测微管相关蛋白2(MAP-2)和生长相关蛋白43(GAP-43)的表达,并计数神经元突起的长度和数目。结果培养细胞均高表达神经元特异性标记物MAP-2。白藜芦醇组细胞活力较对照组明显增加(0.551±0.009比0.436±0.013,P<0.01),而凋亡则明显降低(18.3%±1.3%比35.3%±1.9%,P<0.01),上调MAP-2(0.790±0.102比0.462±0.063,P<0.01)和GAP-43(0.768±0.084比0.424±0.065,P<0.01)蛋白的表达,增加神经元突起的长度(89.510±6.939比61.538±9.14,P<0.01)和数目(6.347±1.002比3.040±0.608,P<0.01)。结论白藜芦醇预处理能减轻氧糖剥夺/再复氧对神经元的损伤,促进神经元突起的生长。Objective To investigate the effects of resveratrol pretreatment on neurite growth of rat primary cortical neurons after oxygen-glucose deprivation/reperfusion（OGD/R） injury in vitro.Methods Primary cortical neurons were cultured under oxygen and glucose deprivation for 150 minutes and reoxygenation for 24 hours.The study had the normal,control and 5μmol/L resveratrol pretreatment groups.Neurons were identified with immunofluorescence.Cell viability was detected with cell counting kit-8（CCK-8） assay.Cell apoptosis was detected with TUNEL assay.Immunofluorescence and Western blotting measured the expressions of microtubule-associated protein 2（MAP-2） and growth associated protein 43（GAP-43）,and the length and number of neurites were counted.Results Cells had high expression of neuronal specific marker MAP-2.Compared with the control group, resveratrol treatment significantly enhanced the neurons viability（0.551 ± 0.009 vs 0.436 ± 0.013,P 〈 0.01）,decreased the numbers of apoptosis（18.3% ± 1.3% vs 35.3% ± 1.9%,P 〈 0.01）,upregulated the expressions of MAP-2（0.790 ± 0.102 vs 0.462 ± 0.063,P 〈 0.01） and GAP-43（0.768 ±0.084 vs 0.424 ± 0.065,P 〈 0.01） proteins,increased the length（89.510 ± 6.939 vs 61.538 ± 9.14,P 〈 0.01） and numbers（6.347 ± 1.002 vs 3.040 ± 0.608,P 〈 0.01） of neurites.Conclusion Resveratrol pretreatment can reduce injury and promote neurite growth of cultured neurons after OGD/R.

关 键 词：白藜芦醇 神经保护 神经突起 氧糖剥夺/再复氧 免疫印迹法 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] R845.22[医药卫生—基础医学]